Tan E, Low K W, Wong W S F, Leung K Y
School of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.
Department of Pharmacology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.
Microbiology (Reading). 1998 Feb;144 ( Pt 2):299-307. doi: 10.1099/00221287-144-2-299.
Aeromonas hydrophila is a Gram-negative bacterium that is pathogenic in fish, causing motile aeromonad septicaemia. It can enter (invade) fish cells, and survive as an intracellular parasite. The host-pathogen interaction and signal transduction pathway were studied by screening signal transduction inhibitors using carp epithelial cells and a virulent strain of the bacterium, PPD134/91. Genistein, a tyrosine kinase inhibitor, postponed internalization of A. hydrophila into host cells, suggesting that tyrosine phosphorylation plays a role in internalization. In contrast, staurosporine, a protein kinase C inhibitor, and sodium orthovanadate, a protein tyrosine phosphatase inhibitor, accelerated internalization of PPD134/91. Other virulent strains of A. hydrophila were also examined and it is likely that all strains, irrespective of serogroup, use the same signalling pathway to facilitate bacterial uptake.
嗜水气单胞菌是一种革兰氏阴性菌，可在鱼类中致病，引发运动性气单胞菌败血症。它能够进入（侵袭）鱼类细胞，并作为细胞内寄生虫存活。通过使用鲤鱼上皮细胞和该细菌的强毒株PPD134/91筛选信号转导抑制剂，对宿主-病原体相互作用和信号转导途径进行了研究。酪氨酸激酶抑制剂染料木黄酮可延缓嗜水气单胞菌内化进入宿主细胞，这表明酪氨酸磷酸化在内化过程中发挥作用。相比之下，蛋白激酶C抑制剂星形孢菌素和蛋白酪氨酸磷酸酶抑制剂原钒酸钠则加速了PPD134/91的内化。还对嗜水气单胞菌的其他强毒株进行了检测，所有菌株，无论血清群如何，可能都使用相同的信号通路来促进细菌摄取。
