Aimar C
Laboratoire d'Immunologie Comparée, Université Pierre et Marie Curie, Paris, France.
Dev Growth Differ. 1997 Dec;39(6):693-704. doi: 10.1046/j.1440-169x.1997.t01-5-00005.x.
Polyclonal antibodies (IS1) reacting specifically with plasma membrane proteins of the Xenopus oocyte were used to study the formation of new plasma membrane in cleavage furrows. Membrane precursors were detected in the inner cytoplasm, then under the plasma membrane of the animal hemisphere and finally on the furrow's edges. Cycloheximide and colchicine caused abnormal distribution of stained material. IS1 antibodies conjugated to colloidal gold, were used to examine the local insertion of membrane precursors into the furrow region by electron microscopy. Membrane precursors were only detected in intracytoplasmic vesicles that fused with the plasma membrane at the edges of the furrow walls. Arrays of microtubules may guide membrane precursors to the site of their insertion in the furrow walls.
使用与非洲爪蟾卵母细胞质膜蛋白特异性反应的多克隆抗体（IS1）来研究卵裂沟中新质膜的形成。在内质中检测到膜前体，然后在动物半球的质膜下，最后在沟的边缘检测到。放线菌酮和秋水仙碱导致染色物质分布异常。将与胶体金偶联的IS1抗体用于通过电子显微镜检查膜前体在沟区域的局部插入。仅在与沟壁边缘的质膜融合的胞质内小泡中检测到膜前体。微管阵列可能将膜前体引导至其在沟壁中的插入位点。
