Bösch S, Siavoshian S, Jacquot C, Tomasoni C, Dabouis G, Elanbaloussi Y, Leneel T, More M T, Roussakis C
ISOMER (Institut Substances et Organismes de la MER), Faculté de Pharmacie, Nantes, France.
Anticancer Res. 1997 Nov-Dec;17(6D):4595-8.
The correlation between the degree of expression of the multidrug resistance-1 (MDR-1) gene and the process of differentiation into non-small-cell, bronchopulmonary carcinoma was studied in vitro and in vivo. For this purpose, a technique for the quantitative analysis of MDR-1 gene expression was developed by competitive reverse-transcriptase polymerase chain reaction. The study of 9 epidermoid carcinomas with various degrees of differentiation did not enable us to establish a correlation in vivo in the patient. However, an in vitro study performed on a non-small-cell lung carcinoma cell line and two of its clones showed that MDR-1 gene expression increased with the degree of differentiation, which was confirmed in vivo when this line was xenografted into nude mice.
在体外和体内研究了多药耐药-1（MDR-1）基因的表达程度与非小细胞支气管肺癌分化过程之间的相关性。为此，通过竞争性逆转录聚合酶链反应开发了一种用于定量分析MDR-1基因表达的技术。对9例不同分化程度的表皮样癌进行的研究未能使我们在患者体内建立相关性。然而，对一种非小细胞肺癌细胞系及其两个克隆进行的体外研究表明，MDR-1基因表达随分化程度增加，当该细胞系异种移植到裸鼠体内时，这一点在体内得到了证实。
