Hui A B, Cheung S T, Fong Y, Lo K W, Huang D P
Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, Chinese University of Hong Kong, Shatin, N.T., People's Republic of China.
Cancer Genet Cytogenet. 1998 Mar;101(2):83-8. doi: 10.1016/s0165-4608(97)00231-8.
Only a few reports exist of cytogenetic studies in nasopharyngeal carcinoma (NPC), probably because of the technical limitations of culturing solid tumors. We established a cell line (cell-666) that was derived from an NPC xenograft growing in athymic nude mice. Epstein-Barr virus (EBV) infection is one of the important etiologic factors of NPC. This cell line has been shown to have the consistent presence of EBV by Epstein-Barr virus encoded RNAs (EBER) in situ hybridization. Furthermore, aberrant methylation of 5'CpG island of p16 gene was detected. Thus, this cell line represents a valuable model for the study of NPC tumorigenecity. The karyotype of this newly established cell line is: 45,X,del(X)(q24),-5der(5)(5pter-->5q31::5q13-->+ ++5q31::5p12-->5pter),der(6;?) (q10;p10),add(7)(p22), + 9, del(11)(q13),add(12)(p13),-14,-18,-21, + 2mar. Fluorescence in situ hybridization analyses were performed using whole chromosome painting probes of chromosomes 5, 6, 11, and 12.
关于鼻咽癌（NPC）细胞遗传学研究的报道很少，可能是因为实体瘤培养存在技术限制。我们建立了一个细胞系（cell-666），它源自无胸腺裸鼠体内生长的鼻咽癌异种移植瘤。爱泼斯坦-巴尔病毒（EBV）感染是鼻咽癌的重要病因之一。通过爱泼斯坦-巴尔病毒编码RNA（EBER）原位杂交已证明该细胞系中持续存在EBV。此外，还检测到p16基因5'CpG岛的异常甲基化。因此，该细胞系是研究鼻咽癌肿瘤发生的有价值模型。这个新建立的细胞系的核型为：45,X,del(X)(q24),-5der(5)(5pter→5q31::5q13→+++5q31::5p12→5pter),der(6;?)(q10;p10),add(7)(p22),+9,del(11)(q13),add(12)(p13),-14,-18,-21,+2mar。使用染色体5、6、11和12的全染色体涂染探针进行了荧光原位杂交分析。
