Kumar V, Goguen D M, Guido M E, Rusak B
Department of Psychology, Dalhousie University, Halifax, NS, Canada.
Brain Res Mol Brain Res. 1997 Dec 15;52(2):242-8. doi: 10.1016/s0169-328x(97)00260-x.
We have assessed whether melatonin can induce c-fos expression at various circadian phases, and whether melatonin can inhibit photically induced c-fos expression in the suprachiasmatic nucleus (SCN) in both rats and Syrian hamsters. Subcutaneous administration of melatonin at a dose of 100 microg/kg neither induced expression of Fos, the protein product of the c-fos proto-oncogene, nor inhibited the expression of Fos-like immunoreactivity (Fos-lir) induced by a light pulse in the SCN of rats and hamsters. In situ hybridization studies also demonstrated the absence of induction by acute melatonin treatments of c-fos mRNA in the SCN. Taken together, these results demonstrate that melatonin effects on SCN cells involve signal transduction pathways that do not include regulation of c-fos gene expression.
我们评估了褪黑素是否能在不同昼夜节律阶段诱导c-fos表达，以及褪黑素是否能抑制大鼠和叙利亚仓鼠视交叉上核（SCN）中光诱导的c-fos表达。以100微克/千克的剂量皮下注射褪黑素，既不会诱导c-fos原癌基因的蛋白质产物Fos的表达，也不会抑制大鼠和仓鼠SCN中光脉冲诱导的Fos样免疫反应性（Fos-lir）的表达。原位杂交研究也表明，急性褪黑素处理不会在SCN中诱导c-fos mRNA。综上所述，这些结果表明褪黑素对SCN细胞的作用涉及不包括c-fos基因表达调控的信号转导途径。
