Shev S, Björkman P, Norkrans G, Foberg U, Frydén A, Lindh G, Lindholm A, Weiland O, Widell A
Department of Infectious Diseases, Sahlgrenska University Hospital/Ostra, Göteborg, Sweden.
J Med Virol. 1998 Feb;54(2):75-9. doi: 10.1002/(sici)1096-9071(199802)54:2<75::aid-jmv1>3.0.co;2-l.
Sera from 62 hepatitis C virus (HCV)-infected Swedish blood donors were tested by a nested polymerase chain reaction using primers targeting the 5'-noncoding region of the GB virus-C/hepatitis G (GBV-C/HGV) genome and an enzyme-linked immunosorbent assay that detects antibodies to the envelope protein E2 of GBV-C/HGV (anti-E2). Fourteen (22%) and 21 (34%) of the 62 blood donors were found to be GBV-C/HGV RNA and anti-E2 positive, respectively. None of the blood donors was positive for both GBV-C/HGV RNA and anti-E2. Thus, 35 of 62 (56%) HCV-infected donors had been exposed to GBV-C/HGV infection. At sequencing of the 14 GBV-C/HGV isolates, 12 were identified as subtype 2a and 2 as subtype 2b. One of 7 (14%) donors with mild liver disease such as steatosis and nonspecific reactive hepatitis had been exposed to GBV-C/HGV vs. 34 of 55 (62%) with chronic hepatitis with or without cirrhosis (P = 0.04). All other differences in histology were small between HCV and dual HCV GBV-C/HGV-infected donors. In conclusion, more than half of HCV-infected Swedish blood donors in this study were positive for either GBV-C/HGV RNA or anti-E2. GBV-C/HGV viremia and seropositivity were mutually exclusive.
使用靶向GB病毒C型/庚型肝炎病毒（GBV-C/HGV）基因组5'非编码区的引物，通过巢式聚合酶链反应以及一种检测GBV-C/HGV包膜蛋白E2抗体（抗-E2）的酶联免疫吸附测定法，对62名感染丙型肝炎病毒（HCV）的瑞典献血者的血清进行了检测。62名献血者中分别有14名（22%）和21名（34%）被发现GBV-C/HGV RNA和抗-E2呈阳性。没有一名献血者GBV-C/HGV RNA和抗-E2均呈阳性。因此，62名HCV感染的献血者中有35名（56%）曾接触过GBV-C/HGV感染。在对14株GBV-C/HGV分离株进行测序时，12株被鉴定为2a亚型，2株为2b亚型。7名患有轻度肝病（如脂肪变性和非特异性反应性肝炎）的献血者中有1名（14%）曾接触过GBV-C/HGV，而55名患有慢性肝炎（伴有或不伴有肝硬化）的献血者中有34名（62%）曾接触过GBV-C/HGV（P = 0.04）。在HCV感染和HCV与GBV-C/HGV双重感染的献血者之间，组织学上的所有其他差异都很小。总之，在本研究中，超过一半的HCV感染瑞典献血者GBV-C/HGV RNA或抗-E2呈阳性。GBV-C/HGV病毒血症和血清阳性是相互排斥的。
