Menegola E, Broccia M L, Prati M, Giavini E
Department of Biology, University of Milan, Italy.
Int J Dev Biol. 1998 Jan;42(1):99-102.
In this work we study the skeletal teratogenic response in rats exposed to NaVP at different embryonic stages. Crl:CD female rats were treated subcutaneously with 400 mg/Kg b.w. NaVP at presomitic stage (group II) or nearly at 2, 6, 10, 14, 18 or 22 somites (groups III-VIII). The females on group I were treated with saline and served as controls. No treatment-related effects were observed at the level of resorptions, live fetuses and fetal or placental weight. The skeletal examination showed characteristic patterns of malformations strictly related to the period of treatment. In particular, groups II and III showed a significant increase of alterations of cervical vertebrae (mainly 1st to 3rd segment) and a decrease of the frequency of extra lumbar ribs in comparison to control. Group IV showed severe abnormalities localized at the 4th to 7th cervical segment and at the level of the 1st and 2nd thoracic segments, including duplications of thoracic segments 1, 2 or 3. The fetuses of group V were characterized by several alterations of the thoracic segments distributed without a clear specificity. In group VI, the thoracic region was also affected with some specificity at the level of the segments 4th to 9th; in group VII, last thoracic and lumbar segments were affected (mainly duplications) and in group VIII only lumbo-sacral abnormalities were recorded. These results confirm the specific effect of NaVP at the level of the axial skeleton and suggest a possible interaction with the expression of genes identifying the vertebral segments.
在本研究中，我们探究了在不同胚胎阶段暴露于戊烯醇钠（NaVP）的大鼠的骨骼致畸反应。将Crl:CD雌性大鼠在体节形成前阶段皮下注射400 mg/Kg体重的NaVP（II组），或在大约2、6、10、14、18或22体节时注射（III - VIII组）。I组雌性大鼠用生理盐水处理作为对照。在吸收、活胎以及胎儿或胎盘重量方面未观察到与处理相关的影响。骨骼检查显示出与处理时期严格相关的特征性畸形模式。具体而言，与对照组相比，II组和III组显示颈椎改变（主要是第1至3节段）显著增加，额外腰椎肋骨频率降低。IV组显示严重异常集中在第4至7颈椎节段以及第1和第2胸椎节段水平，包括第1、2或3胸椎节段的重复。V组胎儿的特征是胸椎节段有多种改变，分布无明显特异性。在VI组中，胸椎区域在第4至9节段水平也受到一些特异性影响；在VII组中，最后胸椎和腰椎节段受到影响（主要是重复），而在VIII组中仅记录到腰骶部异常。这些结果证实了NaVP在轴骨骼水平的特定作用，并提示其可能与确定椎骨节段的基因表达存在相互作用。
