Rhoton-Vlasak A, Wagner J M, Rutgers J L, Baergen R N, Young R H, Roche P C, Plummer T B, Gleich G J
Department of Immunology, Mayo Clinic and Mayo Foundation, Rochester, MN 55905, USA.
Hum Pathol. 1998 Mar;29(3):280-8. doi: 10.1016/s0046-8177(98)90048-x.
Placental site trophoblastic tumor (PSTT) consists of a neoplastic proliferation of intermediate or extravillous trophoblast (also known as X cells). Pregnancy-associated major basic protein (pMBP) is a marker for placental intermediate trophoblast. We compared the distribution of pMBP and human placental lactogen (hPL) in 24 PSTT and 3 exaggerated placental site (EPS) specimens using two distinct immunohistologic methods. Statistical analyses were used to compare staining intensities in metastatic and nonmetastatic lesions. By immunofluorescence, 77% of the PSTT specimens and 100% of the EPS specimens stained with antibodies to pMBP, and the pMBP was localized in intermediate trophoblast and surrounding extracellular areas. By immunohistochemistry, 78% of the PSTT specimens and 100% of the EPS specimens stained for pMBP with a pattern comparable with that of immunofluorescence. Likewise, by immunohistochemistry, hPL stained 96% of the PSTT specimens and 100% of the EPS specimens. Immunohistochemical staining intensities for pMBP and hPL correlated (r2 = +.24; P = .013), but hPL staining was mainly confined to intermediate trophoblast and was more intense. Anti-pMBP tended to stain metastatic PSTT weakly. Thus, pMBP is a useful marker for intermediate trophoblast tumors and could help distinguish these from other forms of trophoblastic disease.
胎盘部位滋养细胞肿瘤（PSTT）由中间型或绒毛外滋养细胞（也称为X细胞）的肿瘤性增殖构成。妊娠相关主要碱性蛋白（pMBP）是胎盘中间型滋养细胞的一个标志物。我们使用两种不同的免疫组织学方法比较了24例PSTT和3例超常胎盘部位（EPS）标本中pMBP和人胎盘催乳素（hPL）的分布情况。采用统计学分析比较转移灶和非转移灶中的染色强度。通过免疫荧光法，77%的PSTT标本和100%的EPS标本用抗pMBP抗体染色，且pMBP定位于中间型滋养细胞及周围细胞外区域。通过免疫组织化学法，78%的PSTT标本和100%的EPS标本pMBP染色，其模式与免疫荧光法相当。同样，通过免疫组织化学法，hPL对96%的PSTT标本和100%的EPS标本染色。pMBP和hPL的免疫组织化学染色强度具有相关性（r2 = +.24；P = .013），但hPL染色主要局限于中间型滋养细胞且更强。抗pMBP对转移性PSTT的染色往往较弱。因此，pMBP是中间型滋养细胞肿瘤的一个有用标志物，有助于将其与其他形式的滋养细胞疾病区分开来。
