Xue Y, Smedts F, Debruyne F M, de la Rosette J J, Schalken J A
Department of Urology, University Hospital Nijmegen, The Netherlands.
Prostate. 1998 Mar 1;34(4):292-301. doi: 10.1002/(sici)1097-0045(19980301)34:4<292::aid-pros7>3.0.co;2-j.
The secretory acini of the adult human prostate contain basal, luminal, and intermediate types of exocrine cells. Intermediate cells are thought to play an important role in normal growth and neoplastic transformation. In this study we investigated whether this cell type is present in early stages of prostate development, using keratin antibodies specific for them.
Autoptic tissue from 11 prepubertal and 5 normal adult prostates was immunohistochemically stained with four keratin antibodies capable of specifically detecting basal, luminal, or intermediate cell types.
Morphologically, in fetal prostate cells differentiation was often not evident. However, basally located cells usually displayed a basal-cell keratin-phenotype. Morphologically similar cells with more luminal localization expressed keratins typical of luminal cells, or of intermediate cells.
成年男性前列腺的分泌腺泡包含基底细胞、管腔细胞和中间型外分泌细胞。中间型细胞被认为在正常生长和肿瘤转化中起重要作用。在本研究中，我们使用针对中间型细胞的角蛋白抗体，研究这种细胞类型是否存在于前列腺发育的早期阶段。
对11例青春期前和5例正常成年前列腺的尸检组织，用四种能够特异性检测基底细胞、管腔细胞或中间型细胞类型的角蛋白抗体进行免疫组织化学染色。
在形态学上，胎儿前列腺细胞的分化通常不明显。然而，位于基底的细胞通常表现出基底细胞角蛋白表型。形态学上相似但定位更靠近管腔的细胞表达管腔细胞或中间型细胞典型的角蛋白。
1）在前列腺发育的早期阶段，可以识别出具有中间型角蛋白表型的细胞。2）它们的大量存在符合从基底细胞到管腔细胞的细胞分化层次途径。3）在胎儿如此早期阶段存在中间型细胞可能反映了它们在前列腺发育中的调节功能。
