Draguhn A, Pfeiffer M, Heinemann U, Polder R
Institut für Physiologie der Charité, Berlin, Germany.
J Neurosci Methods. 1997 Dec 30;78(1-2):105-13. doi: 10.1016/s0165-0270(97)00138-6.
A new hardware cell model for electrophysiological recording has been constructed which allows for the assessment of voltage clamp accuracy in different recording situations. Each compartment consists of a capacitor in parallel with a variable resistor and can be connected to other compartments by a variable axial resistance. The simulated membrane resistance can be changed extrinsically by a command voltage input which is optically coupled to the cell without any direct galvanic contact. Each compartment possesses a buffer amplifier which reads out the potential at the simulated membrane element, (e.g. 'somatic' or 'dendritic' potential). The model allows for the direct observation of typical situations and problems arising in electrophysiological experiments. We used the model to monitor deviations between the 'intracellular' and the command voltage, e.g. due to series resistance errors. We also used the model to simulate synaptic currents which were generated by triangular membrane conductance changes. The results demonstrate the strong influence of synaptic location and series resistance on voltage clamp fidelity. The cell model is a new and easy-to-handle tool for the observation of voltage control under realistic experimental conditions.
已构建了一种用于电生理记录的新型硬件细胞模型，该模型可在不同记录情况下评估电压钳的准确性。每个隔室由一个与可变电阻并联的电容器组成，并可通过可变轴向电阻与其他隔室相连。模拟膜电阻可通过与细胞进行光耦合的指令电压输入从外部进行改变，而无需任何直接的电接触。每个隔室都有一个缓冲放大器，用于读出模拟膜元件处的电位（例如“胞体”或“树突”电位）。该模型可直接观察电生理实验中出现的典型情况和问题。我们使用该模型监测“细胞内”电压与指令电压之间的偏差，例如由于串联电阻误差导致的偏差。我们还使用该模型模拟由三角形膜电导变化产生的突触电流。结果表明突触位置和串联电阻对电压钳保真度有很大影响。该细胞模型是一种用于在实际实验条件下观察电压控制的新型且易于操作的工具。
