Quintana J G, Lopez-Colberg I, Cunningham L A
Department of Neurosciences, University of New Mexico School of Medicine, Albuquerque 87131, USA.
Brain Res Dev Brain Res. 1998 Jan 14;105(1):147-51.
Embryonic ventral midbrains from GFAP-lacZ transgenic mice were xenografted into the dopamine-depleted striata of adult rats. This transgenic line harbors a nuclear-targeted bacterial beta-galactosidase (beta-gal) reporter gene under transcriptional control of the human glial fibrillary acidic protein (GFAP) promoter sequence. Five weeks post-transplantation, graft-derived astrocytes and dopaminergic neurons were visualized by dual immunocytochemistry for beta-gal and tyrosine hydroxylase (TH), respectively. This report describes the advantages associated with the use of GFAP-lacZ transgenic mice to study astrocyte fate in embryonic neural grafts.
将来自GFAP-lacZ转基因小鼠的胚胎腹侧中脑移植到成年大鼠多巴胺耗竭的纹状体中。该转基因品系在人胶质纤维酸性蛋白（GFAP）启动子序列的转录控制下，含有一个核靶向细菌β-半乳糖苷酶（β-gal）报告基因。移植后五周，通过分别针对β-gal和酪氨酸羟化酶（TH）的双重免疫细胞化学法观察移植来源的星形胶质细胞和多巴胺能神经元。本报告描述了使用GFAP-lacZ转基因小鼠研究胚胎神经移植中星形胶质细胞命运的相关优势。
