一种基于快速逆转录聚合酶链反应的BCR-ABL易位检测方法。
A rapid RT-PCR based method for the detection of BCR-ABL translocation.
作者信息
Saltykova L B, Lyschov A A, Abdulkadyrov K M, Blinov M N
机构信息
Institute of Haematology and Transfusiology, St Petersburg, Russia.
出版信息
Mol Pathol. 1997 Oct;50(5):266-8. doi: 10.1136/mp.50.5.266.
AIMS
To optimise a one step reverse transcriptase polymerase chain reaction (RT-PCR) protocol for BCR-ABL chimaera detection.
METHODS
Compared with published RT-PCR procedures, this novel approach has at least two advantages. First, the same enzyme is used for both reverse transcription and PCR. Second, amplification of the target (BCR-ABL chimaera) and control gene (ABL) is performed simultaneously in the same tube.
RESULTS
On testing 40 chronic myelogenous leukaemia patients and 10 healthy donors there was a specificity for the newly developed technique. In addition, dilution experiments demonstrated that the protocol was highly sensitive.
CONCLUSIONS
The suggested one step PCR strategy is a simple and reliable way to reveal BCR-ABL chimaeras.
目的
优化一种用于检测BCR-ABL嵌合体的一步法逆转录聚合酶链反应（RT-PCR）方案。
方法
与已发表的RT-PCR方法相比，这种新方法至少有两个优点。第一，逆转录和PCR使用相同的酶。第二，在同一管中同时进行靶标（BCR-ABL嵌合体）和对照基因（ABL）的扩增。
结果
对40例慢性粒细胞白血病患者和10名健康供体进行检测，新开发的技术具有特异性。此外，稀释实验表明该方案具有高度敏感性。
结论
所建议的一步法PCR策略是一种简单可靠的检测BCR-ABL嵌合体的方法。
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