Kauhanen S, von Boguslawsky K, Michelsson J E, Leivo I
IV Department of Surgery, Helsinki University Central Hospital, Finland.
Acta Neuropathol. 1998 Feb;95(2):165-70. doi: 10.1007/s004010050782.
The time course and pattern of mitotic activity in the deep part of the vastus intermedius muscle (VIP) immobilized in a shortened position in 16 adult NZW rabbit hindlimbs for time periods ranging from 12 h to 56 days and in VIP muscles from 10 rabbit hindlimbs immobilized from 3 to 28 days and subsequently remobilized for 4 weeks were compared. Paraffin sections were stained immunohistochemically with monoclonal MIB 1 antibody raised against recombinant fragments of Ki-67 antigen, and a double staining with modified reticulin stain was carried out to facilitate differentiation between satellite cells and other proliferating cells outside the muscle. In samples taken 24 h after the onset of immobilization, the tissue was mitotically quiescent and at 3, 14, 28, and 56 days of immobilization 5.7%, 1.3%, 1.1% and 0.7%, respectively, of all counted nuclei were mitotically active. However, all of them were localized outside the basement membrane (BM) of the myofiber and were presumably proliferating fibroblasts, capillary endothelial cells or inflammatory cells. Satellite cell mitotic activity was first seen after 2 weeks of immobilization followed by 4 weeks of remobilization. In VIP muscle immobilized for 3 weeks and remobilized for 4 weeks the numbers of all proliferating cells as well as proliferating satellite cells reached a concomitant peak, and they were 7.5% and 3% respectively. The results indicate a late activation of satellite cell proliferation, which may relate to the initial lack of stretch and mechanical load during the cast immobilization followed by a late release of mitogenic factors during eccentric muscle work performed by the VIP muscle after removal of the cast.
比较了16只成年新西兰白兔后肢股中间肌深部（VIP）在缩短位置固定12小时至56天期间的有丝分裂活动的时间进程和模式，以及10只兔后肢VIP肌肉在固定3至28天随后再活动4周期间的有丝分裂活动的时间进程和模式。石蜡切片用针对Ki-67抗原重组片段产生的单克隆MIB 1抗体进行免疫组织化学染色，并进行改良网状纤维染色的双重染色，以促进卫星细胞与肌肉外其他增殖细胞之间的区分。在固定开始后24小时采集的样本中，组织处于有丝分裂静止状态，在固定3天、14天、28天和56天时，所有计数细胞核中有丝分裂活跃的分别占5.7%、1.3%、1.1%和0.7%。然而，所有这些细胞都位于肌纤维基底膜（BM）之外，推测是增殖的成纤维细胞、毛细血管内皮细胞或炎性细胞。卫星细胞的有丝分裂活动在固定2周后，随后再活动4周时首次出现。在固定3周并再活动4周的VIP肌肉中，所有增殖细胞以及增殖卫星细胞的数量同时达到峰值，分别为7.5%和3%。结果表明卫星细胞增殖的激活较晚，这可能与石膏固定期间最初缺乏拉伸和机械负荷有关，随后在去除石膏后VIP肌肉进行离心肌肉工作期间有丝分裂原因子的后期释放有关。
