Chen D, Waite L C, Pierce W M
Department of Pharmacology and Toxicology, University of Louisville School of Medicine, KY 40292, USA.
Biol Trace Elem Res. 1998 Jan;61(1):9-18. doi: 10.1007/BF02784036.
The addition of physiological concentrations of zinc (25-200 (microg/dL) to Dulbecco's Modified Eagle's Medium containing tibiae from 19-d chick embryos resulted in a concentration-dependent increase in tibial content of tartrate-resistant acid phosphatase (TRAP) and an increase in bone resorption, as measured by tibial calcium release. This increase in bone resorption was additive to the resorptive effect resulting from the addition of 10(-9)-10(-7) M parathyroid hormone (PTH), but was not additive to similar effects produced by the addition of 10(-9)-10(-7) M prostaglandin E2 (PGE2). An inhibitor of prostaglandin synthesis, flurbiprofen (10[-6] M), did not influence the effect of zinc on bone resorption. However, the addition of 2,6-pyridinedicarboxylic acid (10[-3] M, 2,6-PDCA), a chelator of zinc, did attenuate the effects of zinc, as did the addition of an inhibitor of DNA replication (hydroxyurea, 10[-3] M). Hydroxyurea also attenuated the bone resorptive response to PGE2, but had no influence on the effects of PTH. These results indicate that physiological concentrations of zinc alter bone resorptive rates in vitro by a mechanism that is dependent on DNA replication.
向含有19日龄鸡胚胫骨的杜尔贝科改良伊格尔培养基中添加生理浓度的锌（25 - 200微克/分升），导致抗酒石酸酸性磷酸酶（TRAP）的胫骨含量呈浓度依赖性增加，并且骨吸收增加，这通过胫骨钙释放来衡量。这种骨吸收的增加与添加10⁻⁹ - 10⁻⁷ M甲状旁腺激素（PTH）所产生的吸收作用相加，但与添加10⁻⁹ - 10⁻⁷ M前列腺素E2（PGE2）所产生的类似作用不相加。前列腺素合成抑制剂氟比洛芬（10⁻⁶ M）不影响锌对骨吸收的作用。然而，添加锌螯合剂2,6 - 吡啶二甲酸（10⁻³ M，2,6 - PDCA）确实减弱了锌的作用，添加DNA复制抑制剂（羟基脲，10⁻³ M）也有同样效果。羟基脲也减弱了对PGE2的骨吸收反应，但对PTH的作用没有影响。这些结果表明，生理浓度的锌通过一种依赖于DNA复制的机制在体外改变骨吸收速率。
