Yorde D E, Sasse E A, Wang T Y, Hussa R O, Garancis J C
Clin Chem. 1976 Aug;22(8):1372-7.
We described the principle of a new enzyme-immunoassay, competitive enzyme-liked immunoassay (CELIA), for quantitative measurement of soluble antigens and haptens. In the assay, binding of antibody to antigen-immunosorbent is competitively inhibited by the free antigen to be measured. The amount of first antibody bound to the immunosorbent is measured by an enzymatic technique in which a heterologous bridging antibody and a soluble antibody/enzyme immune complex are applied in sequence. The soluble complex we used was rabbit antiperoxidase/horseradish peroxidase. Peroxidase activity is inversely proportional to the concentration in the original sample of the substance to be assayed. The enzyme-linked reagents are potentially widely applicable to any substance to be measured. To demonstrate the feasibility of CELIA, we report a preliminary study of its application to the measurement of human chloriogonadotropin in serum and urine. The assay described for this hormone has a working range of 1 to 50 int. units per milliliter of sample. The technique obviates the disadvantages associated with measurement and handling of radioisotopes in radioimmunoassays and the only major instrumentation required is a centrifuge and a conventional spectrophotometer.
我们描述了一种用于定量检测可溶性抗原和半抗原的新型酶免疫测定法——竞争性酶联免疫测定法（CELIA）的原理。在该测定法中，待检测的游离抗原竞争性抑制抗体与抗原免疫吸附剂的结合。通过一种酶促技术测定与免疫吸附剂结合的一抗的量，该技术依次应用异源桥连抗体和可溶性抗体/酶免疫复合物。我们使用的可溶性复合物是兔抗过氧化物酶/辣根过氧化物酶。过氧化物酶活性与待分析物质原始样品中的浓度成反比。酶联试剂可能广泛适用于任何待测量的物质。为证明CELIA的可行性，我们报告了其应用于血清和尿液中人绒毛膜促性腺激素测量的初步研究。针对该激素描述的测定法的工作范围为每毫升样品1至50国际单位。该技术避免了放射免疫测定中与放射性同位素测量和处理相关的缺点，并且所需的唯一主要仪器是离心机和传统分光光度计。
