Durantel D, Croizier G, Ravallec M, López-Ferber M
Unité de Génétique des Virus, INRA-URA CNRS 2209, Saint Christol-les-Alès, France.
Virology. 1998 Feb 15;241(2):276-84. doi: 10.1006/viro.1997.8971.
Autographa californica nucleopolyhedrovirus (AcMNPV) lef-4 gene [ORF 90; Ayres et al. (1994) Virology 202, 586-605] is involved in both late and very late gene expression [Passarelli and Miller (1993) Virology 197, 704-714]. The transcriptional properties of this gene have been analyzed. It is transcribed as a single 1.6-kb mRNA and transcripts were first detected 3 h postinfection (pi). The extremities of the transcript have been mapped by primer extension and 3' RACE-PCR to positions -56 from the translation start codon and +96 downstream of the stop codon. A rabbit polyclonal antiserum has been raised against an internal polypeptide of LEF-4. A 55-kDa protein was observed by Western blot analysis from 5 h pi. LEF-4 localizes preferentially in the nucleus of infected cells and is associated with the virogenic stroma.
苜蓿银纹夜蛾核型多角体病毒（AcMNPV）的lef-4基因[开放阅读框90；艾尔斯等人（1994年），《病毒学》202, 586 - 605]参与晚期和极晚期基因表达[帕萨雷利和米勒（1993年），《病毒学》197, 704 - 714]。该基因的转录特性已得到分析。它转录为一条单一的1.6千碱基的信使核糖核酸（mRNA），转录本在感染后3小时首次被检测到。转录本的末端已通过引物延伸和3'端快速扩增cDNA末端聚合酶链反应（3' RACE-PCR）定位到翻译起始密码子上游56个碱基处以及终止密码子下游96个碱基处。已制备了针对LEF-4内部多肽的兔多克隆抗血清。通过蛋白质免疫印迹分析在感染后5小时观察到一种55千道尔顿的蛋白质。LEF-4优先定位于受感染细胞的细胞核中，并与病毒发生基质相关。
