Méndez A, Martín de las Mulas J, Bautista M J, Chacón F, Millán Y, Fondevila D, Pumarola M
Department of Comparative Pathology, Veterinary School, University of Córdoba, Spain.
J Comp Pathol. 1998 Jan;118(1):29-40. doi: 10.1016/s0021-9975(98)80025-x.
The presence and distribution of S100 protein (alpha and beta subunits), cytokeratin polypeptides, glial fibrillary acidic protein, neurofilaments, vimentin, neuron specific enolase, synaptophysin, HLA class II DR antigen, and pituitary hormones (prolactin, adrenocorticotropic hormone and human chorionic gonadotrophin) in stellate cells were studied immunohistochemically in four normal canine pituitary glands, five canine pituitary adenomas, two canine pituitary carcinomas and two equine pituitary adenomas (with surrounding normal glandular tissue). Stellate cells of the pars distalis and pars intermedia of canine and equine adenohypophyses showed a strong reaction with antibodies against S100 protein subunits alpha and beta. They also reacted with antibody against high and low molecular weight cytokeratins, but not with those against other intermediate filament proteins, neuroendocrine markers, the HLA-class II DR antigen or the pituitary hormones. Other populations of cells expressing both subunits of the S100 protein were polygonal cells of the pars distalis of the adenohypophysis (horse) and marginal epithelial cells of the pars intermedia of the adenohypophysis (dog and horse). Some pituitary tumours had S100-immunoreactive cells with a distribution of alpha and beta subunits that differed between the two species. Some canine tumours (one adenoma and one carcinoma) expressed only the alpha subunit, but both of the equine adenomas expressed alpha and beta protein subunits. Some of the S100-immunoreactive tumour cells reacted with RCK-102 (cytokeratins 5+8) antibody in the dog but not in the horse. The results suggested that canine and equine stellate cells of the adenohypophysis are more closely related to epithelial than to glial cells, as is the case in cattle, sheep and goats but not human beings or mice. No subpopulation of cells of bone marrow origin could be identified among canine stellate cells, as they lack MHC class II antigen. The results also suggested that the presence of S100-immunoreactive cells is more striking in canine and equine tumours than in human tumours.
采用免疫组织化学方法，对4个正常犬垂体、5个犬垂体腺瘤、2个犬垂体癌和2个马垂体腺瘤（及其周围正常腺组织）中的星状细胞进行研究，以观察S100蛋白（α和β亚基）、细胞角蛋白多肽、胶质纤维酸性蛋白、神经丝、波形蛋白、神经元特异性烯醇化酶、突触素、HLA-II类DR抗原和垂体激素（催乳素、促肾上腺皮质激素和人绒毛膜促性腺激素）的存在及分布情况。犬和马腺垂体远侧部和中间部的星状细胞与抗S100蛋白亚基α和β的抗体呈强反应。它们也与抗高分子量和低分子量细胞角蛋白的抗体发生反应，但与抗其他中间丝蛋白、神经内分泌标志物、HLA-II类DR抗原或垂体激素的抗体无反应。其他表达S100蛋白两个亚基的细胞群体是腺垂体远侧部（马）的多边形细胞和腺垂体中间部（犬和马）的边缘上皮细胞。一些垂体肿瘤有S100免疫反应性细胞，其α和β亚基的分布在两个物种之间有所不同。一些犬肿瘤（1个腺瘤和1个癌）仅表达α亚基，但两个马腺瘤均表达α和β蛋白亚基。一些S100免疫反应性肿瘤细胞在犬中与RCK-102（细胞角蛋白5+8）抗体反应，但在马中无反应。结果表明，犬和马腺垂体的星状细胞与上皮细胞的关系比与神经胶质细胞更为密切，牛、羊和山羊的情况也是如此，但人类和小鼠并非如此。在犬星状细胞中未发现骨髓来源的细胞亚群，因为它们缺乏MHC-II类抗原。结果还表明，S100免疫反应性细胞在犬和马肿瘤中的存在比在人类肿瘤中更为显著。
