Fuxa JR, Richter AR
Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, 70803, USA.
J Invertebr Pathol. 1998 Mar;71(2):159-64. doi: 10.1006/jipa.1997.4724.
A laboratory population of Anticarsia gemmatalis subjected to 80% mortality by the A. gemmatalis nucleopolyhedrovirus (AgNPV) developed a 5x resistance ratio (RR) within four generations. When exposure to AgNPV was discontinued, the resistant insects returned to their original level of susceptibility within three generations. Two additional cycles of resistance selection and reversion were repeated in the same insect population with similar results, except that the insects reached a 5x RR within three generations in the second cycle and two generations in the third. The insects returned to the original level of susceptibility within two generations in both the second and third resistance/reversion cycles. The resistant insects produced 61% as many viable neonates as susceptible insects. Resistant insects also had longer life spans, a lower rate of larval survival in rearing, and lower pupal weights than susceptible insects. These differences in life histories, all significant at P < 0.01, indicate that A. gemmatalis resistance to AgNPV might have associated costs in fitness. The addition of an optical brightener (OB) to the AgNPV suspension increased the susceptibility of resistant and susceptible insects by 24x and 58x, respectively, resulting in an increase of the RR from 4.7 to 11.2x. Thus, the LC50 of AgNPV with an optical brightener against resistant insects was less than the LC50 of AgNPV without brightener against susceptible insects. Copyright 1998 Academic Press.
用斜纹夜蛾核型多角体病毒（AgNPV）处理斜纹夜蛾实验室种群，使其死亡率达到80%，该种群在四代内产生了5倍的抗性比（RR）。当停止接触AgNPV后，抗性昆虫在三代内恢复到原来的敏感水平。在同一昆虫种群中重复进行了另外两个抗性选择和恢复周期，结果相似，只是在第二个周期中昆虫在三代内达到了5倍的RR，在第三个周期中两代内达到了5倍的RR。在第二个和第三个抗性/恢复周期中，昆虫均在两代内恢复到原来的敏感水平。抗性昆虫产生的存活幼虫数量仅为敏感昆虫的61%。抗性昆虫的寿命也更长，饲养过程中幼虫存活率较低，蛹重也低于敏感昆虫。这些生活史差异在P < 0.01时均具有显著性，表明斜纹夜蛾对AgNPV的抗性可能伴随着适合度代价。在AgNPV悬浮液中添加荧光增白剂（OB），分别使抗性和敏感昆虫的敏感性提高了24倍和58倍，导致RR从4.7倍增加到11.2倍。因此，添加荧光增白剂的AgNPV对抗性昆虫的LC50低于未添加增白剂的AgNPV对敏感昆虫的LC50。版权所有1998年学术出版社。
