Reyes R A, Cockerell G L
Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523-1671, USA.
Virology. 1998 Mar 1;242(1):184-92. doi: 10.1006/viro.1998.9029.
To further investigate the molecular basis underlying the dysregulation of B cell homeostasis associated with bovine leukemia virus disease progression in cattle, bovine bax was cDNA cloned and sequenced. The predicted amino acid sequence of bovine Bax revealed a 192-amino-acid protein having extensive identity with the human (97%), murine (93%), and rat (94%) homologues. Because the ratio of Bcl-2 to Bax is believed to predetermine the susceptibility to a given apoptotic stimulus, the relative expression of the genes encoding these oncoproteins was evaluated in cattle naturally infected with BLV. In BLV-infected cattle an increase in the ratios of bcl-2/bax mRNA and protein expression correlated with advancing stages of disease. These findings suggest that in addition to the maintenance of BLV-associated hematopoietic malignancies, the reciprocal expression of Bcl-2/Bax may modulate the induction of B cell expansion typical of BLV disease progression.
为了进一步研究牛白血病病毒疾病进展过程中与牛B细胞稳态失调相关的分子基础，对牛bax进行了cDNA克隆和测序。预测的牛Bax氨基酸序列显示，其为一种含有192个氨基酸的蛋白质，与人类（97%）、小鼠（93%）和大鼠（94%）的同源物具有广泛的同源性。由于据信Bcl-2与Bax的比例预先决定了对给定凋亡刺激的易感性，因此在自然感染牛白血病病毒的牛中评估了编码这些癌蛋白的基因的相对表达。在感染牛白血病病毒的牛中，bcl-2/bax mRNA和蛋白表达的比率增加与疾病进展阶段相关。这些发现表明，除了维持与牛白血病病毒相关的造血系统恶性肿瘤外，Bcl-2/Bax的相互表达可能调节牛白血病病毒疾病进展典型的B细胞扩增诱导。
