用于分离和鉴定位点特异性蛋白酶的遗传筛选。
A genetic screen for the isolation and characterization of site-specific proteases.
作者信息
Sices H J, Kristie T M
机构信息
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 4-Room 133, 9000 Rockville Pike, Bethesda, MD 20892, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):2828-33. doi: 10.1073/pnas.95.6.2828.
Abstract
Site-specific proteolysis is an important regulatory mechanism in basic cellular and viral processes. Using the protease of the HIV as a model, a genetic system has been developed for the isolation and characterization of site-specific proteases. The system utilizes the well defined bacteriophage lambda regulatory circuit where the viral repressor, cI, is specifically cleaved to initiate the lysogenic-to-lytic switch. The model system is rapid, highly specific, and demonstrates the ability to isolate and characterize enzymes of limited expression or activity. In addition, the system has a significant potential for the selection of clinically relevant mutant enzymes and in the development of anti-protease therapeutics.
摘要
位点特异性蛋白水解是基础细胞和病毒过程中的一种重要调控机制。以HIV蛋白酶为模型，已开发出一种用于分离和表征位点特异性蛋白酶的遗传系统。该系统利用了定义明确的噬菌体λ调控回路，其中病毒阻遏物cI被特异性切割以启动溶原到裂解的转换。该模型系统快速、高度特异，并展示了分离和表征低表达或低活性酶的能力。此外，该系统在选择临床相关突变酶和开发抗蛋白酶疗法方面具有巨大潜力。
相似文献
1
A genetic screen for the isolation and characterization of site-specific proteases.用于分离和鉴定位点特异性蛋白酶的遗传筛选。
Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):2828-33. doi: 10.1073/pnas.95.6.2828.
2
Synthetic protease switch.合成蛋白酶开关
Science. 1998 Apr 17;280(5362):456.
3
Rapid genetic selection of inhibitor-resistant protease mutants: clinically relevant and novel mutants of the HIV protease.
AIDS Res Hum Retroviruses. 2001 Sep 1;17(13):1249-55. doi: 10.1089/088922201750461302.
4
A bacteriophage lambda-based genetic screen for characterization of the activity and phenotype of the human immunodeficiency virus type 1 protease.一种基于噬菌体λ的遗传筛选方法，用于鉴定人类免疫缺陷病毒1型蛋白酶的活性和表型。
Antimicrob Agents Chemother. 2000 May;44(5):1132-9. doi: 10.1128/AAC.44.5.1132-1139.2000.
5
Bacteriophage λ RexA and RexB functions assist the transition from lysogeny to lytic growth.噬菌体 λ 的 RexA 和 RexB 功能有助于从溶原状态到裂解生长的转变。
Mol Microbiol. 2021 Oct;116(4):1044-1063. doi: 10.1111/mmi.14792. Epub 2021 Aug 30.
6
Action at a distance in CI repressor regulation of the bacteriophage 186 genetic switch.噬菌体186遗传开关的CI阻遏物调控中的远程作用。
Mol Microbiol. 2002 Aug;45(3):697-710. doi: 10.1046/j.1365-2958.2002.03038.x.
7
The OR control system of bacteriophage lambda. A physical-chemical model for gene regulation.噬菌体λ的OR控制系统。基因调控的物理化学模型。
J Mol Biol. 1985 Jan 20;181(2):211-30. doi: 10.1016/0022-2836(85)90086-5.
8
Cro's role in the CI Cro bistable switch is critical for {lambda}'s transition from lysogeny to lytic development.Cro在CI Cro双稳态开关中的作用对于λ从溶原性向裂解发育的转变至关重要。
Genes Dev. 2007 Oct 1;21(19):2461-72. doi: 10.1101/gad.1584907.
9
Quantitative model for gene regulation by lambda phage repressor.λ噬菌体阻遏物基因调控的定量模型。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1129-33. doi: 10.1073/pnas.79.4.1129.
10
The Developmental Switch in Bacteriophage λ: A Critical Role of the Cro Protein.噬菌体λ中的发育开关：Cro蛋白的关键作用。
J Mol Biol. 2018 Jan 5;430(1):58-68. doi: 10.1016/j.jmb.2017.11.005. Epub 2017 Nov 20.
引用本文的文献
1
Epistasis as a determinant of the HIV-1 protease's robustness to mutation.上位性作为HIV-1蛋白酶对突变的稳健性的一个决定因素。
PLoS One. 2014 Dec 31;9(12):e116301. doi: 10.1371/journal.pone.0116301. eCollection 2014.
2
A tobacco etch virus protease with increased substrate tolerance at the P1' position.一种烟草蚀纹病毒蛋白酶，其在 P1'位置对底物的耐受性增强。
PLoS One. 2013 Jun 24;8(6):e67915. doi: 10.1371/journal.pone.0067915. Print 2013.
3
Fitness landscape of human immunodeficiency virus type 1 protease quasispecies.人类免疫缺陷病毒1型蛋白酶准种的适应性景观
J Virol. 2007 Mar;81(5):2485-96. doi: 10.1128/JVI.01594-06. Epub 2006 Dec 6.
4
HIV-1 protease catalytic efficiency effects caused by random single amino acid substitutions.随机单氨基酸取代导致的HIV-1蛋白酶催化效率影响。
Mol Biol Evol. 2007 Feb;24(2):382-7. doi: 10.1093/molbev/msl168. Epub 2006 Nov 7.
5
HIV protease-activated molecular switches based on beta-glucuronidase and alkaline phosphatase.基于β-葡萄糖醛酸酶和碱性磷酸酶的HIV蛋白酶激活分子开关
Comb Chem High Throughput Screen. 2006 May;9(4):313-20. doi: 10.2174/138620706776843219.
6
Genetic screen for monitoring severe acute respiratory syndrome coronavirus 3C-like protease.用于监测严重急性呼吸综合征冠状病毒3C样蛋白酶的基因筛选
J Virol. 2004 Dec;78(24):14057-61. doi: 10.1128/JVI.78.24.14057-14061.2004.
7
Model system for high-throughput screening of novel human immunodeficiency virus protease inhibitors in Escherichia coli.用于在大肠杆菌中高通量筛选新型人类免疫缺陷病毒蛋白酶抑制剂的模型系统。
Antimicrob Agents Chemother. 2004 Jul;48(7):2437-47. doi: 10.1128/AAC.48.7.2437-2447.2004.
8
Genetic screen for monitoring hepatitis C virus NS3 serine protease activity.用于监测丙型肝炎病毒NS3丝氨酸蛋白酶活性的基因筛选
Antimicrob Agents Chemother. 2003 May;47(5):1760-5. doi: 10.1128/AAC.47.5.1760-1765.2003.
9
A yeast-based growth assay for the analysis of site-specific proteases.一种用于分析位点特异性蛋白酶的基于酵母的生长测定法。
Nucleic Acids Res. 2003 Feb 15;31(4):e16. doi: 10.1093/nar/gng016.
10
Rapid assessment of phenotypic resistance to protease inhibitors in human immunodeficiency virus type 1 group O.1型人类免疫缺陷病毒O组对蛋白酶抑制剂表型耐药性的快速评估
J Clin Microbiol. 2002 Nov;40(11):4313-6. doi: 10.1128/JCM.40.11.4313-4316.2002.
本文引用的文献
1
Death substrates come alive.死亡底物“复活”了。
Bioessays. 1997 Jun;19(6):501-7. doi: 10.1002/bies.950190609.
2
Regulation of transcription factor function by proteolysis.通过蛋白水解作用对转录因子功能的调控。
Biochem Soc Trans. 1997 May;25(2):498-502. doi: 10.1042/bst0250498.
3
Presenilins: genes for life and death.早老素：关乎生死的基因。
Neuron. 1997 May;18(5):687-90. doi: 10.1016/s0896-6273(00)80309-8.
4
The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor.固醇调节元件结合蛋白（SREBP）途径：通过膜结合转录因子的蛋白水解作用调节胆固醇代谢
Cell. 1997 May 2;89(3):331-40. doi: 10.1016/s0092-8674(00)80213-5.
5
Human immunodeficiency virus type 1 protease inhibitors.人类免疫缺陷病毒1型蛋白酶抑制剂
Arch Intern Med. 1997 May 12;157(9):951-9.
6
Cleavage site for sterol-regulated protease localized to a leu-Ser bond in the lumenal loop of sterol regulatory element-binding protein-2.固醇调节蛋白酶的切割位点定位于固醇调节元件结合蛋白2腔环中的亮氨酸-丝氨酸键处。
J Biol Chem. 1997 May 9;272(19):12778-85. doi: 10.1074/jbc.272.19.12778.
7
Substrate and inhibitor specificity of interleukin-1 beta-converting enzyme and related caspases.白细胞介素-1β转换酶及相关半胱天冬酶的底物和抑制剂特异性
J Biol Chem. 1997 Mar 14;272(11):7223-8. doi: 10.1074/jbc.272.11.7223.
8
Internally consistent libraries of fluorogenic substrates demonstrate that Kex2 protease specificity is generated by multiple mechanisms.荧光底物的内部一致文库表明，Kex2蛋白酶的特异性是由多种机制产生的。
Biochemistry. 1997 Feb 18;36(7):1912-7. doi: 10.1021/bi961779l.
9
DCP-1, a Drosophila cell death protease essential for development.DCP-1，一种对果蝇发育至关重要的细胞死亡蛋白酶。
Science. 1997 Jan 24;275(5299):536-40. doi: 10.1126/science.275.5299.536.
10
Mutational anatomy of an HIV-1 protease variant conferring cross-resistance to protease inhibitors in clinical trials. Compensatory modulations of binding and activity.一种在临床试验中对蛋白酶抑制剂具有交叉耐药性的HIV-1蛋白酶变体的突变剖析。结合与活性的补偿性调节。
J Biol Chem. 1996 Dec 13;271(50):31957-63. doi: 10.1074/jbc.271.50.31957.
Zotero 插件