足月分娩时人蜕膜中前列腺素内过氧化物合酶-1和-2的mRNA水平及酶活性

Prostaglandin endoperoxide H synthase-1 and -2 mRNA levels and enzyme activity in human decidua at term labor.

作者信息

Hirst J J, Mijovic J E, Zakar T, Olson D M

机构信息

Department of Obstetrics and Gynaecology, University of Alberta, Edmonton, Canada.

出版信息

J Soc Gynecol Investig. 1998 Jan-Feb;5(1):13-20. doi: 10.1016/s1071-5576(97)00101-9.

DOI:10.1016/s1071-5576(97)00101-9

PMID:9501293

Abstract

OBJECTIVE

To determine the labor-related changes of prostaglandin endoperoxide H synthase (PGHS) activity and PGHS-1 and -2 abundance in term decidua and to assess the contribution of the PGHS isoforms to the total PGHS activity present in the tissue.

METHODS

Decidua was collected after elective cesarean delivery (CD) or spontaneous labor (SL) at term. Prostaglandin endoperoxide H synthase activity was determined in microsomal fractions, and PGHS-1 and -2 mRNA levels were measured by ribonuclease protection assays. Prostaglandin endoperoxide H synthase-1 and -2 mRNAs were localized in tissue sections by in situ hybridization.

RESULTS

Prostaglandin endoperoxide H synthase specific activity in decidua microsomes at CD was 111 +/- 3 pg prostaglandin-E2/minute/microgram protein (mean +/- standard error, N = 10 patients), not different from enzyme activity measured after SL (110 +/- 27 N = 10 patients, P = .97, Wilcoxon's rank sum test). Prostaglandin endoperoxide H synthase-1 mRNA abundance in CD tissues was 0.283 +/- 0.047 relative densitometric units (mean +/- standard error, n = 26 patients), which did not change with labor (SL: 0.329 +/- 0.073, n = 20 patients, P = .68). Prostaglandin endoperoxide H synthase-2 mRNA abundance was also unaffected by labor (CD: 0.933 +/- 0.255, n = 27 patients; SL: 0.714 +/- 0.179, n = 23 patients, mean +/- standard error, P = .66). Prostaglandin endoperoxide H synthase specific activity was positively and significantly (P < .05) correlated with both PGHS-1 and -2 mRNA levels. In situ hybridization showed the pervasive presence of both PGHS mRNAs in decidua cells with no detectable changes associated with labor.

CONCLUSION

Both isoforms of PGHS are present in term decidua and contribute to enzyme activity and prostaglandin production. Mechanisms regulating decidual prostanoid biosynthesis at labor do not involve changing the levels of expression of the two PGHS isoforms.

摘要

目的

确定足月蜕膜中前列腺素内过氧化物合酶（PGHS）活性以及PGHS-1和PGHS-2丰度与分娩相关的变化，并评估PGHS同工型对组织中总PGHS活性的贡献。

方法

在足月择期剖宫产（CD）或自然分娩（SL）后收集蜕膜。测定微粒体部分的前列腺素内过氧化物合酶活性，并用核糖核酸酶保护分析法测量PGHS-1和PGHS-2 mRNA水平。通过原位杂交将前列腺素内过氧化物合酶-1和-2 mRNA定位在组织切片中。

结果

剖宫产时蜕膜微粒体中前列腺素内过氧化物合酶比活性为111±3 pg前列腺素-E2/分钟/微克蛋白质（平均值±标准误，N = 10例患者），与自然分娩后测得的酶活性无差异（110±27，N = 10例患者，P = 0.97，Wilcoxon秩和检验）。剖宫产组织中前列腺素内过氧化物合酶-1 mRNA丰度为0.283±0.047相对光密度单位（平均值±标准误，n = 26例患者），不随分娩而改变（自然分娩：0.329±0.073，n = 20例患者，P = 0.68）。前列腺素内过氧化物合酶-2 mRNA丰度也不受分娩影响（剖宫产：0.933±0.255，n = 27例患者；自然分娩：0.714±0.179，n = 23例患者，平均值±标准误，P = 0.66）。前列腺素内过氧化物合酶比活性与PGHS-1和PGHS-2 mRNA水平均呈显著正相关（P < 0.05）。原位杂交显示蜕膜细胞中两种PGHS mRNA均普遍存在，与分娩无关的可检测变化。