针对血型糖蛋白C和D的新型鼠单克隆抗体具有抗Ge2特异性。
New murine monoclonal antibodies directed against glycophorins C and D, have anti-Ge2 specificity.
作者信息
Janvier D, Veaux S, Benbunan M
机构信息
Etablissement de Transfusion Sanguine de l'Assistance Publique, Hôpitaux de Paris, Site St-Louis, France.
出版信息
Vox Sang. 1998;74(2):101-5.
BACKGROUND AND OBJECTIVES
The Gerbich blood group system comprises three high-incidence antigens which have been localized on glycophorins C (GPC) and D (GPD) molecules. Murine monoclonal antibodies (mAbs) directed against Ge4 and Ge3 epitopes have already been obtained. We describe two murine mAbs (ZI51.17 and J6) with Ge2 specificity.
MATERIALS AND METHODS
The mAbs were investigated by serological methods and immunoblotting, with common, Ge-negative and enzyme-modified RBCs.
RESULTS
Both mAbs behaved like anti-Ge2 in serological tests, but bound to GPC and GPD on immunoblots. ZI51.17 did not recognize the GPCYus or GPCGe variants, whereas J6 revealed a 25-kD component from Ge:-2,-3,4 membranes, which is identical to the GPCGe molecule.
CONCLUSION
These two mAbs are directed to slightly different epitopes. The ZI51.17 and J6 epitopes are common to GPC and GPD, thus distinct from the Ge2 antigen defined by human alloantibodies.
背景与目的
杰尔比希血型系统包含三种高频率抗原，这些抗原已定位在血型糖蛋白C（GPC）和D（GPD）分子上。针对Ge4和Ge3表位的鼠单克隆抗体（mAb）已获得。我们描述了两种具有Ge2特异性的鼠单克隆抗体（ZI51.17和J6）。
材料与方法
采用血清学方法和免疫印迹法，用普通、Ge阴性及酶修饰的红细胞对单克隆抗体进行研究。
结果
两种单克隆抗体在血清学检测中表现为抗Ge2，但在免疫印迹中与GPC和GPD结合。ZI51.17不识别GPCYus或GPCGe变体，而J6显示来自Ge:-2,-3,4细胞膜的一个25-kD成分，它与GPCGe分子相同。
结论
这两种单克隆抗体针对的是略有不同的表位。ZI51.17和J6表位是GPC和GPD共有的，因此不同于人同种抗体所定义的Ge2抗原。
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