Roelcke D, Hack H, Kreft H, Gross H J
Institute for Immunology, University of Heidelberg, Germany.
Vox Sang. 1998;74(2):109-12.
Pr1,2,3, PrM, Sa and Sia-l1, -b1, -lb1 are sialic acid (NeuNAc)-dependent antigens recognized by human cold agglutinins. Pr and Sa antigens are the O-glycans of glycophorins containing alpha 2,3NeuNAc (to galactose) and/or alpha 2,6NeuNAc (to galactosamine). The antigens of the Sia-l1, -b1, -lb1 complex are gangliosides that may carry alpha 2,3NeuNAc (to galactose) and/or alpha 2,8NeuNAc (to NeuNAc). We studied the NeuNAc groups involved in the antigens.
From 74 sera with cold agglutinins against NeuNAc-dependent antigens, anti-T-free preparations were made and tested against human red cells, treated with an alpha 2,3-specific recombinant sialidase.
Most (51/62) Pr antigens use alpha 2,3NeuNAc, some (8/62) use alpha 2,6NeuNAc and a few (3/62) use both sialyl groups as immunodominant components on glycophorins. The immunodominant component of Sa and Sia-l1, -b1, -lb1 determinants was alpha 2,3NeuNAc in all cases.
The red cell target structures for cold agglutinins against NeuNAc-dependent antigens have been identified. We propose a Pr nomenclature to reflect this. The binding of anti-Pr to gangliosides may be the basis for anti-Pr-induced peripheral neuropathy.
Pr1,2,3、PrM、Sa以及Sia-l1、-b1、-lb1是人类冷凝集素识别的唾液酸（NeuNAc）依赖性抗原。Pr和Sa抗原是血型糖蛋白的O-聚糖，含有α2,3NeuNAc（连接到半乳糖）和/或α2,6NeuNAc（连接到N-乙酰半乳糖胺）。Sia-l1、-b1、-lb1复合物的抗原是神经节苷脂，可能携带α2,3NeuNAc（连接到半乳糖）和/或α2,8NeuNAc（连接到NeuNAc）。我们研究了参与这些抗原的NeuNAc基团。
从74份针对NeuNAc依赖性抗原的冷凝集素血清中制备了抗T-free制剂，并对用α2,3特异性重组唾液酸酶处理过的人红细胞进行检测。
大多数（51/62）Pr抗原使用α2,3NeuNAc，一些（8/62）使用α2,6NeuNAc，少数（3/62）同时使用这两种唾液酸基团作为血型糖蛋白上的免疫显性成分。在所有情况下，Sa和Sia-l1、-b1、-lb1决定簇的免疫显性成分都是α2,3NeuNAc。
已鉴定出针对NeuNAc依赖性抗原的冷凝集素的红细胞靶结构。我们提出一种Pr命名法来反映这一点。抗Pr与神经节苷脂的结合可能是抗Pr诱导的周围神经病变的基础。
