通过连续色谱法纯化小鼠干扰素。
Purification of mouse interferon by sequential chromatography.
作者信息
Braude I A, De Clercq E
出版信息
J Chromatogr. 1979 Apr 21;172:207-19. doi: 10.1016/s0021-9673(00)90956-7.
Two schemes for the purification of mouse interferon are described, based on the concerted application of various physicochemical and affinity/adsorption column chromatographic techniques. Mouse interferon was purified to a final specific activity of 1.0--8.0 x 10(8) units/mg when first precipitated with ammonium sulphate and further processed by hydrophobic chromatography and adsorption chromatography on AFFI-Gel 202 and Controlled Pore Glass. It was purified to a final specific activity of 2.5--3.7 x 10(8) units/mg when first precipitated with ammonium sulphate and further processed by gel filtration with Ultrogel AcA 54, ion-exchange chromatography with Carboxymethyl Boi-Gel Agarose, hydrophobic chromatography with AFFI-Gel 202 and adsorption chromatography with Controlled Pore Glass.
本文描述了两种纯化小鼠干扰素的方案，这些方案基于多种物理化学方法以及亲和/吸附柱色谱技术的协同应用。当先用硫酸铵沉淀，然后通过疏水色谱以及在AFFI-Gel 202和可控孔径玻璃上进行吸附色谱进一步处理时，小鼠干扰素被纯化至最终比活性为1.0--8.0 x 10(8) 单位/毫克。当先用硫酸铵沉淀，然后通过用Ultrogel AcA 54进行凝胶过滤、用羧甲基琼脂糖凝胶进行离子交换色谱、用AFFI-Gel 202进行疏水色谱以及用可控孔径玻璃进行吸附色谱进一步处理时，其被纯化至最终比活性为2.5--3.7 x 10(8) 单位/毫克。
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