Vincentelli A, Zegdi R, Prat A, Lajos P, Latrémouille C, LeBret E, De Boisbaudry G, Carpentier A, Fabiani J N
Laboratoires des prothèses et greffes cardiaques, Hôpital Broussais, Paris, France.
J Heart Valve Dis. 1998 Jan;7(1):24-9.
The use of human pericardium pretreated for 10 min with 0.625% glutaraldehyde (GLUT) in valvular repair or intracardiac reconstruction has produced good results. However, to date, no investigations have been made to determine the mechanical changes that occur in the tissue following such pretreatment.
Human pericardial samples were harvested from 25 patients and immersed in GLUT for increasing times (5, 10, 30, 60 min and 6 months). Either untreated human pericardium or bovine pericardium treated for six months with GLUT served as controls. Tensile tests were performed with a uniaxial load machine and a pulsative bench. Fatigue testing was for 14 days; each sample was tested at 1,200 cycles/min at a controlled pressure of 90-120 mmHg.
Untreated tissue thickness was 0.44+/-0.16 mm, but after six months GLUT treatment it was 0.53+/-0.15 mm (p<0.001). There was a 13.7% shrinkage of tissue after six months immersion. Strain was significantly greater in treated tissue than in untreated tissue, while stiffness decreased with the duration of GLUT immersion. Young's modulus was significantly lower after six months GLUT treatment (0.26+/-0.06 MPa) compared with untreated, and 5-, 10- and 30-min GLUT treatment (0.32+/-0.15, 0.35+/-0.09, 0.32+/-0.09 and 0.36+/-0.10 MPa (p<0.05)), respectively. Creep was greater after six months GLUT treatment (0.5+/-0.03%) than in untreated and 10-, 30- and 60-min treatments (0.3+/-0.50, 0.27+/-0.01, 0.27+/-0.02, 0.3+/-0.01% (p<0.05)), respectively. Ultimate tensile stress (UTS) was greater in 10-min treated pericardium than in untreated tissue: 38.46+/-11.75 versus 22.17+/-8.30 MPa (p<0.05) respectively. Strain at rupture was greater in the 6-month group (30.62+/-2.54%) than for untreated and 10-, 30- and 60-min GLUT immersion 16.3+/-0.73, 21.85+/-0.75, 20.12+/-1.04 and 18.87+/-0.86% (p<0.05), respectively. Fatigue testing showed an increased length after five and 10 min, and six months, with a lengthening of 14.66, 12.53, 7.66%, respectively compared with 3.5% for untreated tissue (p<0.05). There were three failures in the untreated group (n = 5), none in the 5- and 10-min groups, and one in the 6-month group (p<0.05).
Brief immersion of human pericardial tissue in 0.625% glutaraldehyde reduces the tissue's stiffness and improves its durability for use in cardiac surgery.
在瓣膜修复或心脏内重建中，使用经0.625%戊二醛（GLUT）预处理10分钟的人心包已取得良好效果。然而，迄今为止，尚未进行研究以确定这种预处理后组织中发生的机械变化。
从25名患者身上采集人心包样本，并将其浸入GLUT中不同时间（5、10、30、60分钟和6个月）。未处理的人心包或经GLUT处理6个月的牛心包作为对照。使用单轴加载机和脉动试验台进行拉伸试验。疲劳试验为期14天；每个样本在90 - 120 mmHg的控制压力下以1200次/分钟的频率进行测试。
未处理组织厚度为0.44±0.16毫米，但经GLUT处理6个月后为0.53±0.15毫米（p<0.001）。浸泡6个月后组织收缩13.7%。处理后的组织应变显著大于未处理的组织，而刚度随GLUT浸泡时间的延长而降低。与未处理以及GLUT处理5、10和30分钟后的组织相比，经GLUT处理6个月后的杨氏模量显著更低（分别为0.26±0.06兆帕、0.32±0.15、0.35±0.09、0.32±0.09和0.36±0.10兆帕（p<0.05））。经GLUT处理6个月后的蠕变（0.5±0.03%）大于未处理以及GLUT处理10、30和60分钟后的组织（分别为0.3±0.50、0.27±0.01、0.27±0.02、0.3±0.01%（p<0.05））。经10分钟处理的心包的极限拉伸应力（UTS）大于未处理的组织：分别为38.46±11.75与22.17±8.30兆帕（p<0.05）。破裂应变在6个月组（30.62±2.54%）大于未处理以及GLUT浸泡10、30和60分钟后的组织（分别为16.3±0.73、21.85±0.75、20.12±1.04和18.87±0.86%（p<0.05））。疲劳试验显示，在5分钟、10分钟和6个月后长度增加，与未处理组织的3.5%相比，分别延长了14.66%、12.53%、7.66%（p<0.05）。未处理组（n = 5）有3次失效，5分钟和10分钟组无失效，6个月组有1次失效（p<0.05）。
将人心包组织短暂浸入0.625%戊二醛可降低组织刚度并提高其在心脏手术中的耐用性。
