大鼠模型中微创手术后肾上腺皮质反应及局部T淋巴细胞激活模式

Adrenocortical response and regional T-lymphocyte activation patterns following minimally invasive surgery in a rat model.

作者信息

Berguer R, Dalton M, Ferrick D

机构信息

Department of Surgery, University of California Davis, 4301 X Street, Sacramento, CA 95817, USA.

出版信息

Surg Endosc. 1998 Mar;12(3):236-40. doi: 10.1007/s004649900642.

DOI:10.1007/s004649900642

PMID:9502703

Abstract

BACKGROUND

Laparoscopic surgery is associated with less tissue trauma and postoperative pain as well as a more rapid recovery than open surgery. We hypothesized that these factors may result in less immune impairment following laparoscopic surgery.

METHODS

We measured mitogen-induced surface interleukin-2 receptor (IL2R) expression and lymphocyte proliferation in CD4+ and CD8+ T-lymphocytes as well as serum corticosterone levels in rats 24 h following open (OP) and laparoscopic (LAP) fundoplication.

RESULTS

Serum corticosterone levels were lower in LAP vs OP rats (p = 0.02). CD4+ IL2R expression was higher in the blood, but not in the spleen, in LAP vs OP animals (p = 0.02). CD8+ IL2R expression was similar in both groups. Mitogen-induced lymphocyte proliferation was no different in the blood but decreased in the spleen in LAP vs OP rats (p = 0.03).

CONCLUSIONS

Compared to open surgery, laparoscopic fundoplication in the rat results in lower adrenocortical hormone levels and better-preserved T-helper-cell activation in the blood. Lymphocyte proliferation is suppressed in the spleen 24 h after laparoscopic surgery. Minimally invasive surgery may better preserve cell-mediated immunity in the early postoperative period.

摘要

背景

与开放手术相比，腹腔镜手术造成的组织创伤和术后疼痛更小，恢复也更快。我们推测这些因素可能导致腹腔镜手术后免疫损伤更小。

方法

我们在大鼠进行开放（OP）和腹腔镜（LAP）胃底折叠术24小时后，测量丝裂原诱导的CD4 +和CD8 + T淋巴细胞表面白细胞介素-2受体（IL2R）表达、淋巴细胞增殖以及血清皮质酮水平。

结果

与OP组大鼠相比，LAP组大鼠的血清皮质酮水平较低（p = 0.02）。与OP组动物相比，LAP组动物血液中CD4 + IL2R表达较高，但脾脏中无差异（p = 0.02）。两组CD8 + IL2R表达相似。丝裂原诱导的淋巴细胞增殖在血液中无差异，但与OP组大鼠相比，LAP组大鼠脾脏中的增殖减少（p = 0.03）。