在缺血状态下，星形胶质细胞间隙连接保持开放。
Astrocytic gap junctions remain open during ischemic conditions.
作者信息
Cotrina M L, Kang J, Lin J H, Bueno E, Hansen T W, He L, Liu Y, Nedergaard M
机构信息
Department of Cell Biology, New York Medical College, Valhalla, New York 10595, USA.
出版信息
J Neurosci. 1998 Apr 1;18(7):2520-37. doi: 10.1523/JNEUROSCI.18-07-02520.1998.
Abstract
Gap junctions are highly conductive channels that allow the direct transfer of intracellular messengers such as Ca2+ and inositol triphosphate (IP3) between interconnected cells. In brain, astrocytes are coupled extensively by gap junctions. We found here that gap junctions among astrocytes in acutely prepared brain slices as well as in culture remained open during ischemic conditions. Uncoupling first occurred after the terminal loss of plasma membrane integrity. Gap junctions therefore may link ischemic astrocytes in an evolving infarct with the surroundings. The free exchange of intracellular messengers between dying and potentially viable astrocytes might contribute to secondary expansion of ischemic lesions.
摘要
缝隙连接是高传导性通道，可使细胞内信使如Ca2+和肌醇三磷酸（IP3）在相互连接的细胞之间直接传递。在大脑中，星形胶质细胞通过缝隙连接广泛耦合。我们在此发现，急性制备的脑片以及培养物中的星形胶质细胞之间的缝隙连接在缺血条件下仍保持开放。解偶联首先发生在质膜完整性最终丧失之后。因此，缝隙连接可能将正在形成的梗死灶中的缺血星形胶质细胞与周围环境连接起来。垂死的和潜在存活的星形胶质细胞之间细胞内信使的自由交换可能导致缺血性病变的继发性扩展。
相似文献
1
Astrocytic gap junctions remain open during ischemic conditions.在缺血状态下，星形胶质细胞间隙连接保持开放。
J Neurosci. 1998 Apr 1;18(7):2520-37. doi: 10.1523/JNEUROSCI.18-07-02520.1998.
2
Gap junction blockage limits intercellular spreading of astrocytic apoptosis induced by metabolic depression.间隙连接阻断限制了由代谢抑制诱导的星形胶质细胞凋亡的细胞间传播。
J Neurochem. 2005 Aug;94(4):1111-23. doi: 10.1111/j.1471-4159.2005.03241.x.
3
Immunorecognition, ultrastructure and phosphorylation status of astrocytic gap junctions and connexin43 in rat brain after cerebral focal ischaemia.大鼠局灶性脑缺血后星形胶质细胞缝隙连接和连接蛋白43的免疫识别、超微结构及磷酸化状态
Eur J Neurosci. 1998 Jul;10(7):2444-63. doi: 10.1046/j.1460-9568.1998.00253.x.
4
Identification of second messengers that induce expression of functional gap junctions in microglia cultured from newborn rats.鉴定在新生大鼠原代培养小胶质细胞中诱导功能性缝隙连接表达的第二信使。
Brain Res. 2002 Jul 12;943(2):191-201. doi: 10.1016/s0006-8993(02)02621-5.
5
Gap junction-mediated bidirectional signaling between human fetal hippocampal neurons and astrocytes.人胎儿海马神经元与星形胶质细胞之间缝隙连接介导的双向信号传导。
Dev Neurosci. 2001;23(6):420-31. doi: 10.1159/000048729.
6
Opening of astrocytic mitochondrial ATP-sensitive potassium channels upregulates electrical coupling between hippocampal astrocytes in rat brain slices.星形胶质细胞线粒体 ATP 敏感性钾通道的开放上调了大鼠脑片海马星形胶质细胞之间的电耦联。
PLoS One. 2013;8(2):e56605. doi: 10.1371/journal.pone.0056605. Epub 2013 Feb 13.
7
Astrocytic gap junctional networks suppress cellular damage in an in vitro model of ischemia.星形细胞缝隙连接网络抑制体外缺血模型中的细胞损伤。
Biochem Biophys Res Commun. 2014 Feb 7;444(2):171-6. doi: 10.1016/j.bbrc.2014.01.035. Epub 2014 Jan 16.
8
Neuroprotective role of astrocytic gap junctions in ischemic stroke.星形胶质细胞缝隙连接在缺血性卒中中的神经保护作用。
Cell Commun Adhes. 2003 Jul-Dec;10(4-6):413-7. doi: 10.1080/cac.10.4-6.413.417.
9
Metabolic inhibition induces opening of unapposed connexin 43 gap junction hemichannels and reduces gap junctional communication in cortical astrocytes in culture.代谢抑制诱导未对接的连接蛋白43间隙连接半通道开放，并减少培养的皮质星形胶质细胞中的间隙连接通讯。
Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):495-500. doi: 10.1073/pnas.012589799. Epub 2001 Dec 26.
10
Octanol, a gap junction uncoupling agent, changes intracellular [H+] in rat astrocytes.辛醇是一种缝隙连接解偶联剂，可改变大鼠星形胶质细胞内的[H⁺]。
Glia. 1996 Jan;16(1):7-15. doi: 10.1002/(SICI)1098-1136(199601)16:1<7::AID-GLIA2>3.0.CO;2-2.
引用本文的文献
1
Licorice extract and carbenoxolone protect PC12 cells against serum/glucose deprivation-induced apoptosis through modulation of caspase-3 and PARP activation.甘草提取物和甘珀酸通过调节半胱天冬酶-3和聚（ADP-核糖）聚合酶的激活来保护PC12细胞免受血清/葡萄糖剥夺诱导的凋亡。
Avicenna J Phytomed. 2025 Jul-Aug;15(4):1252-1263. doi: 10.22038/ajp.2024.25252.
2
Calcium Role in Gap Junction Channel Gating: Direct Electrostatic or Calmodulin-Mediated?钙在缝隙连接通道门控中的作用：直接静电作用还是钙调蛋白介导的？
Int J Mol Sci. 2024 Sep 10;25(18):9789. doi: 10.3390/ijms25189789.
3
Gap Junction Channel Regulation: A Tale of Two Gates-Voltage Sensitivity of the Chemical Gate and Chemical Sensitivity of the Fast Voltage Gate.缝隙连接通道调控：双门开关的故事—化学门控的电压敏感性和快电压门控的化学敏感性
Int J Mol Sci. 2024 Jan 12;25(2):982. doi: 10.3390/ijms25020982.
4
Targeting Cx43 to Reduce the Severity of Pressure Ulcer Progression.靶向连接蛋白 43 以减轻压疮进展的严重程度。
Cells. 2023 Dec 18;12(24):2856. doi: 10.3390/cells12242856.
5
Ca-dependent rapid uncoupling of astrocytes upon brief metabolic stress.短暂代谢应激时星形胶质细胞依赖钙的快速解偶联
Front Cell Neurosci. 2023 Oct 11;17:1151608. doi: 10.3389/fncel.2023.1151608. eCollection 2023.
6
The Polyamine Spermine Potentiates the Propagation of Negatively Charged Molecules through the Astrocytic Syncytium.多胺精胺增强带负电荷的分子通过星形胶质细胞合胞体的传播。
Biomolecules. 2022 Dec 5;12(12):1812. doi: 10.3390/biom12121812.
7
Crosstalk of Astrocytes and Other Cells during Ischemic Stroke.缺血性中风期间星形胶质细胞与其他细胞的相互作用
Life (Basel). 2022 Jun 17;12(6):910. doi: 10.3390/life12060910.
8
Connexins, Pannexins and Gap Junctions in Perinatal Brain Injury.围产期脑损伤中的连接蛋白、泛连接蛋白与缝隙连接
Biomedicines. 2022 Jun 18;10(6):1445. doi: 10.3390/biomedicines10061445.
9
Calmodulin-Connexin Partnership in Gap Junction Channel Regulation-Calmodulin-Cork Gating Model.钙调蛋白-连接蛋白在缝隙连接通道调节中的伙伴关系-钙调蛋白-软木塞门控模型。
Int J Mol Sci. 2021 Dec 2;22(23):13055. doi: 10.3390/ijms222313055.
10
HIV infection of astrocytes compromises inter-organelle interactions and inositol phosphate metabolism: A potential mechanism of bystander damage and viral reservoir survival.HIV 感染星形胶质细胞会损害细胞器间的相互作用和肌醇磷酸盐代谢：旁观者损伤和病毒储存库存活的潜在机制。
Prog Neurobiol. 2021 Nov;206:102157. doi: 10.1016/j.pneurobio.2021.102157. Epub 2021 Aug 26.
本文引用的文献
1
Intracellular calcium oscillations in astrocytes: a highly plastic, bidirectional form of communication between neurons and astrocytes in situ.星形胶质细胞内的钙振荡：神经元与原位星形胶质细胞之间一种高度可塑性的双向通讯形式。
J Neurosci. 1997 Oct 15;17(20):7817-30. doi: 10.1523/JNEUROSCI.17-20-07817.1997.
2
Impact of cytoplasmic calcium buffering on the spatial and temporal characteristics of intercellular calcium signals in astrocytes.细胞质钙缓冲对星形胶质细胞中细胞间钙信号的时空特征的影响。
J Neurosci. 1997 Oct 1;17(19):7359-71. doi: 10.1523/JNEUROSCI.17-19-07359.1997.
3
Postnatal development of ionic currents in rat hippocampal astrocytes in situ.大鼠海马星形胶质细胞原位离子电流的出生后发育
J Neurophysiol. 1997 Jul;78(1):461-77. doi: 10.1152/jn.1997.78.1.461.
4
Brain damage and temperature: cellular and molecular mechanisms.脑损伤与体温：细胞及分子机制
Adv Neurol. 1996;71:177-94; discussion 194-7.
5
Octanol, a gap junction uncoupling agent, changes intracellular [H+] in rat astrocytes.辛醇是一种缝隙连接解偶联剂，可改变大鼠星形胶质细胞内的[H⁺]。
Glia. 1996 Jan;16(1):7-15. doi: 10.1002/(SICI)1098-1136(199601)16:1<7::AID-GLIA2>3.0.CO;2-2.
6
Hippocampal astrocytes in situ respond to glutamate released from synaptic terminals.原位海马星形胶质细胞对从突触终末释放的谷氨酸作出反应。
J Neurosci. 1996 Aug 15;16(16):5073-81. doi: 10.1523/JNEUROSCI.16-16-05073.1996.
7
Bystander killing of cancer cells by herpes simplex virus thymidine kinase gene is mediated by connexins.单纯疱疹病毒胸苷激酶基因介导旁观者对癌细胞的杀伤作用是由连接蛋白介导的。
Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1831-5. doi: 10.1073/pnas.93.5.1831.
8
In vitro ischemia promotes calcium influx and intracellular calcium release in hippocampal astrocytes.体外缺血促进海马星形胶质细胞内的钙内流和细胞内钙释放。
J Neurosci. 1996 Jan;16(1):71-81. doi: 10.1523/JNEUROSCI.16-01-00071.1996.
9
Gap junctions are required for the propagation of spreading depression.扩散性抑制的传播需要缝隙连接。
J Neurobiol. 1995 Dec;28(4):433-44. doi: 10.1002/neu.480280404.
10
Correlation between peri-infarct DC shifts and ischaemic neuronal damage in rat.大鼠梗死周边去极化漂移与缺血性神经元损伤之间的相关性
Neuroreport. 1993 Jun;4(6):709-11. doi: 10.1097/00001756-199306000-00027.
Zotero 插件