绘制性连锁贫血基因图谱：小鼠肠道铁吸收的一种遗传性缺陷

Mapping the gene for sex-linked anemia: an inherited defect of intestinal iron absorption in the mouse.

作者信息

Anderson G J, Murphy T L, Cowley L, Evans B A, Halliday J W, McLaren G D

机构信息

Clinical Sciences Unit, Queensland Institute of Medical Research, P.O. Royal Brisbane Hospital, Australia.

出版信息

Genomics. 1998 Feb 15;48(1):34-9. doi: 10.1006/geno.1997.5138.

DOI:10.1006/geno.1997.5138

PMID:9503013

Abstract

The sex-linked anemic (sla) mouse carries an anemia that results from an inherited defect of intestinal iron absorption and provides an ideal model with which to investigate this poorly understood yet clinically important process. We have precisely mapped the sla locus within the central region of the X chromosome in relation to a panel of microsatellite markers. Analysis of over 500 progeny from an intraspecific intercross and a smaller intraspecific backcross segregating sla established the following locus order in the sla region: DXMit45-sla- (DXMit16, DXMit96)-DXMit41-DXMit169-DXMit170- DXMit148-(DXMit18, DXMit171)-DXMit84-DXMit64. The two microsatellites DXMit16 and DXMit96 are located 0.60 +/- 0.35cM from sla and form the telomeric limit of the sla region. The mapping of the sla locus is an important first step to identifying the gene itself.

摘要

性连锁贫血（sla）小鼠患有因肠道铁吸收遗传缺陷导致的贫血症，为研究这一尚未完全了解但在临床上很重要的过程提供了理想模型。我们已将sla基因座精确定位在X染色体的中心区域，与一组微卫星标记相关。对来自种内杂交和较小的种内回交的500多个后代进行分析，这些后代分离出sla，从而确定了sla区域的以下基因座顺序：DXMit45-sla-（DXMit16，DXMit96）-DXMit41-DXMit169-DXMit170-DXMit148-（DXMit18，DXMit171）-DXMit84-DXMit64。两个微卫星DXMit16和DXMit96距离sla为0.60 +/- 0.35cM，构成sla区域的端粒边界。sla基因座的定位是鉴定该基因本身的重要第一步。