羽扇豆P0核糖体蛋白的cDNA表达与纯化
Expression of the cDNA and purification of P0 ribosomal protein from Lupinus luteus.
作者信息
Mikołajczyk K, Barciszewski J
机构信息
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
出版信息
Biochem Mol Biol Int. 1998 Jan;44(1):69-77. doi: 10.1080/15216549800201072.
PMID:9503149
Abstract
Eukaryotic ribosomal protein P0 is localized on the large ribosomal subunit at the base of the specific "stalk" structure, close to the central protuberance. Recently we have obtained a clone coding for P0 ribosomal protein from L. luteus (yellow lupin) cDNA library. Here we present its expression in E. coli cells and purification of the target protein.
摘要
真核生物核糖体蛋白P0定位于大核糖体亚基特定“柄”结构的基部，靠近中央突起。最近，我们从黄花羽扇豆（黄羽扇豆）cDNA文库中获得了一个编码P0核糖体蛋白的克隆。在此，我们展示其在大肠杆菌细胞中的表达及目标蛋白的纯化。
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