McLennan A E, Brittain T
School of Biological Sciences, University of Auckland, New Zealand.
Biochem Mol Biol Int. 1998 Jan;44(1):175-83. doi: 10.1080/15216549800201192.
The binding of two strong allosteric effectors (2,3 Diphosphoglycerate D.P.G., and Bezafibrate, Bzf) to both adult and the three human embryonic haemoglobins, either individually or in combination, have been studied in detail. The adult protein exhibits one binding site for D.P.G and two for Bzf. When both effectors are present simultaneously their effects are simply additive. The same qualitative pattern of binding is observed in the case of the three human embryonic haemoglobins, although with different binding constants. The lack of synergism between these effectors and the different binding affinity expressed by these proteins are discussed in terms of the known amino acid sequence differences.
详细研究了两种强变构效应剂（2,3-二磷酸甘油酸，D.P.G.；苯扎贝特，Bzf）单独或联合与成人血红蛋白及三种人类胚胎血红蛋白的结合情况。成人血红蛋白对D.P.G.有一个结合位点，对Bzf有两个结合位点。当两种效应剂同时存在时，它们的效应只是简单相加。三种人类胚胎血红蛋白也观察到了相同的定性结合模式，不过结合常数不同。根据已知的氨基酸序列差异，讨论了这些效应剂之间缺乏协同作用以及这些蛋白质表现出的不同结合亲和力。
