Kitahata S, Okada S
J Biochem. 1976 Mar;79(3):641-8. doi: 10.1093/oxfordjournals.jbchem.a131108.
The acceptor specificity of the transglycosylation reaction of cyclodextrin glycosyltransferase[EC 2.4.1.19] was investigated using various sugars and sugar alcohols. L-Sorbose, D-xylose, and D-galactose, which contain configurational or structural changes relative to the D-glucopyranose unit at positions other than position 1, were also shown to be efficient acceptors in the transglycosylation reaction of this enzyme. It was shown by chemical and enzymatic methods that this enzyme could transfer glycosyl residues only to the C3-hydroxyl group of L-sorbose and C4-hydroxyl group of D-xylose, producing oligosaccharides terminated by 3-O-alpha-D-glucopyranosyl-L-sorbose and 4-O-alpha-D-glucopyranosyl-D-xylose at the reducing ends, respectively.
利用各种糖类和糖醇，研究了环糊精糖基转移酶[EC 2.4.1.19]转糖基化反应的受体特异性。L-山梨糖、D-木糖和D-半乳糖相对于D-吡喃葡萄糖单元在1位以外的位置含有构型或结构变化，它们在该酶的转糖基化反应中也被证明是有效的受体。通过化学和酶学方法表明，该酶只能将糖基残基转移到L-山梨糖的C3-羟基和D-木糖的C4-羟基上，分别在还原端产生以3-O-α-D-吡喃葡萄糖基-L-山梨糖和4-O-α-D-吡喃葡萄糖基-D-木糖终止的寡糖。
