采用电子捕获气相色谱法对脑组织中的普萘洛尔、普萘洛尔二醇和N-去异丙基普萘洛尔进行定量测定。

Quantitative determination of propranolol, propranolol glycol and N-desisopropylpropranolol in brain tissue by electron capture gas chromatography.

作者信息

Sealens D A, Walle T, Privitera P J

出版信息

J Chromatogr. 1976 Jul 21;123(1):185-92. doi: 10.1016/s0021-9673(00)81114-0.

Abstract

A method for the quantitative determination of propranolol and two of its active metabolites, 3-(alpha-naphthoxy)-1,2-propanediol (propranolol glycol) and N-des-isopropylpropranolol, in brain tissue of mice is described. Tissues are homogenized in perchloric acid-acetonitrile. Propranolol and its metabolites are isolated from the supernatant by solvent extraction and separated and detected as their trifluoroacetyl derivatives by electron capture gas chromatography. Chemical structures of the derivatives were confirmed by gas chromatography-mass spectrometry. The electron capture detector response of all three compounds is high, 0.7-2.0 X 10(-16) moles/sec. Brain levels of 10-250 ng/g can be detected of all three compounds with high specificity and good precision.

摘要

本文描述了一种定量测定小鼠脑组织中普萘洛尔及其两种活性代谢产物3-(α-萘氧基)-1,2-丙二醇（普萘洛尔二醇）和N-去异丙基普萘洛尔的方法。组织在高氯酸-乙腈中匀浆。普萘洛尔及其代谢产物通过溶剂萃取从上清液中分离出来，并作为其三氟乙酰衍生物通过电子捕获气相色谱法进行分离和检测。衍生物的化学结构通过气相色谱-质谱法确证。所有三种化合物的电子捕获检测器响应都很高，为0.7 - 2.0×10(-16)摩尔/秒。所有三种化合物在脑内的含量在10 - 250纳克/克时，均可被高特异性和高精度地检测到。