Prévots F, Tolou S, Delpech B, Kaghad M, Daloyau M
Faculté de Pharmacie, Toulouse, France.
FEMS Microbiol Lett. 1998 Feb 15;159(2):331-6. doi: 10.1111/j.1574-6968.1998.tb12879.x.
A 7.275-kb DNA fragment which encodes resistance by abortive infection (Abi+) to bacteriophage was cloned from Lactococcus lactis subsp. cremoris S114. The genetic determinant for abortive infection was subcloned from this fragment. This gene was found to confer a reduction in efficiency of plating and plaque size for prolate-headed bacteriophage phi 53 (group I homology) and for small isometric-headed bacteriophage phi 59 (group III homology). This new gene, termed abiN, is predicted to encode a polypeptide of 178 amino acid residues with a deduced molecular mass of 20,461 Da and an isoelectric point of 4.63. No homology with any previously described genes was found. A probe was used to determine the presence of this gene only in S114 from 31 strains tested.
从乳酸乳球菌乳脂亚种S114中克隆出一个7.275 kb的DNA片段，该片段通过流产感染（Abi+）编码对噬菌体的抗性。从该片段中对流产感染的遗传决定因素进行了亚克隆。发现该基因可使长头噬菌体phi 53（I组同源性）和小等轴头噬菌体phi 59（III组同源性）的平板接种效率和噬菌斑大小降低。这个新基因被命名为abiN，预计编码一个由178个氨基酸残基组成的多肽，推导分子量为20461 Da，等电点为4.63。未发现与任何先前描述的基因有同源性。使用探针仅在测试的31个菌株中的S114中确定该基因的存在。
