Tan S Y, Gewolb I H, Hobbins J C
J Clin Endocrinol Metab. 1976 Aug;43(2):412-8. doi: 10.1210/jcem-43-2-412.
A specific and thoroughly validated competitive protein binding radioassay has been developed for the measurement of unconjugated cortisol in human amniotic fluid. Cortisol levels rose throughout normal gestation from 8.6 +/- 0.8 ng/ml at less than 20 weeks to 19.8 +/- 1.5 ng/ml at 30-40 weeks. Levels were significantly higher in amniotic fluid samples having mature lecithin/sphingomyelin (L/S) ratios (greater than 2), and good correlation with individual cortisol values was obtained (r = 0.79; P less than 0.001). These results support the hypothesis that cortisol plays a role in fetal lung maturation.
已开发出一种特定且经过充分验证的竞争性蛋白结合放射分析方法，用于测定人羊水未结合皮质醇的含量。在整个正常孕期，皮质醇水平不断上升，从孕20周前的8.6±0.8纳克/毫升升至孕30 - 40周时的19.8±1.5纳克/毫升。卵磷脂/鞘磷脂（L/S）比值成熟（大于2）的羊水样本中皮质醇水平显著更高，且与个体皮质醇值具有良好的相关性（r = 0.79；P＜0.001）。这些结果支持了皮质醇在胎儿肺成熟过程中发挥作用这一假说。
