Stasenko A A, Chernyshov V P
Klin Lab Diagn. 1997 Dec(12):22-3, 33.
Study of the functional activity of blood and biliary neutrophils in patients with purulent cholangitis revealed different patterns of blood neutrophil reaction in the NBT test. The share of type 1 cells (with diffuse staining of the cytoplasm) in spontaneous and stimulated NBT test is a constant value in the studied patient population. The count of active blood neutrophils (type 2, with formasane granules) depends on the antigen dose in the test. A more grave form (with strictures of the bile duct) is associated with a decreased count of formasane-positive type 2 cells in comparison with that in patients with choledocholithiasis. An accurate and rapid method for the diagnosis of purulent cholangitis has been developed, based on measuring the functional activity of A bile neutrophils collected during surgery. The C bile neutrophils differ from blood neutrophils of patients with purulent cholangitis by morphology and function. Biliary neutrophils reduce the NBT after the diffuse punctuate type. The detected differences are presumably due to specific features of the environment.
对化脓性胆管炎患者血液和胆汁中性粒细胞功能活性的研究显示，在硝基蓝四氮唑（NBT）试验中血液中性粒细胞反应存在不同模式。在自发和刺激的NBT试验中，1型细胞（细胞质弥漫性染色）的比例在所研究的患者群体中是一个恒定值。活性血液中性粒细胞（2型，有甲臜颗粒）的数量取决于试验中的抗原剂量。与胆总管结石病患者相比，更严重的形式（伴有胆管狭窄）与甲臜阳性2型细胞数量减少有关。基于测量手术中收集的胆汁中性粒细胞的功能活性，已开发出一种准确快速的化脓性胆管炎诊断方法。C胆汁中性粒细胞在形态和功能上与化脓性胆管炎患者的血液中性粒细胞不同。胆汁中性粒细胞在弥漫点状类型后使NBT还原。检测到的差异可能归因于环境的特定特征。
