Harmeyer J, Martens H, Naga M A
J Dairy Sci. 1976 Jul;59(7):1340-1. doi: 10.3168/jds.S0022-0302(76)84365-2.
Growth of a mixed ruminal population taken from a sheep on a protein-free (all urea) purified diet was estimated by an in vitro fermentation technique including precipitation of microbial protein by trichloracetic acid. Volatile fatty acid production in vitro was determined, and the associated a denosine triphosphate was estimated as moles volatile fatty acids X 2.4. On this basis, the quantity of microbial protein synthesized per mole of adenosine triphosphate increased at higher microbial growth rates.
采用体外发酵技术，包括用三氯乙酸沉淀微生物蛋白，对取自以无蛋白（全尿素）纯化日粮饲养的绵羊的混合瘤胃菌群的生长进行了评估。测定了体外挥发性脂肪酸的产生量，并将相关的三磷酸腺苷估计为挥发性脂肪酸的摩尔数×2.4。在此基础上，每摩尔三磷酸腺苷合成的微生物蛋白量在较高的微生物生长速率下增加。
