Spongr V P, Henderson D, McFadden S L
Center for Hearing and Deafness, University at Buffalo, NY 14214, USA.
Scand Audiol Suppl. 1998;48:15-25.
To better understand the nature of mechanical changes following exposure to high-level impact noise, normal cochleas and cochleas from chinchillas exposed to either 125 or 131 dB SPL noise were stained with phalloidin for F-actin and examined using confocal microscopy. As seen in previous experiments, 125 dB exposures produced much more variable results than 131 dB exposures. Some cochleas were relatively unscathed by the exposure, whereas others showed damage to outer hair cells (OHCs) immediately after the exposure that included gross distortions of cell bodies and reduced F-actin in cuticular plates. Twenty-four hours later, there was also disorientation of actin filaments in supporting cells. After 30 days, Deiters cells were disarrayed and cups were separated from OHC neural poles. Exposure to noise at a level of 131 dB SPL produced less variable results than 125 dB exposure, and damage was generally more widespread and severe.
为了更好地理解暴露于高强度冲击噪声后机械变化的本质，对正常的雪貂耳蜗以及暴露于125或131 dB SPL噪声的雪貂耳蜗用鬼笔环肽对F-肌动蛋白进行染色，并使用共聚焦显微镜进行检查。如先前实验所示，125 dB暴露产生的结果比131 dB暴露的结果更具变异性。一些耳蜗在暴露后相对未受损伤，而另一些在暴露后立即显示出外毛细胞（OHC）受损，包括细胞体的严重扭曲和表皮板中F-肌动蛋白减少。24小时后，支持细胞中的肌动蛋白丝也出现紊乱。30天后，Dieters细胞排列紊乱，杯状细胞与OHC神经极分离。与125 dB暴露相比，暴露于131 dB SPL的噪声产生的结果变异性较小，并且损伤通常更广泛、更严重。
