[牛肝酪氨酸-tRNA合成酶多克隆抗体的制备及该酶两种形式的特性分析]
[Production of polyclonal antibodies to tyrosyl-tRNA-synthetase from the bovine liver and characterization of two forms of the enzyme].
作者信息
Rozhko O T, Korneliuk A I, Ribkhinska T A, Savinskaia L N, Odynets K A, Matsuka G Kh
机构信息
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv.
出版信息
Ukr Biokhim Zh (1978). 1997 May-Jun;69(3):9-16.
The polyclonal antibodies purified by affine chromatography against tyrosyl-tRNA synthetase (TyrRS) immobilized on the column with affigel-sepharose have been obtained from the bovine liver. The immunospecificity of these antibodies and their influence on enzymatic activity of TyrRS from the bovine liver have been investigated. We have stated that the polyclonal antibodies inhibited TyrRS enzymatic activity in aminoacylation of homologous tRNA(Tyr) by 47%. As it has been shown by immunoblotting the antibodies reacted both with the native enzyme (M(r) 2.59 kDa) and with the proteolytic cleaved functionally active form of the enzyme (M 2.39 kDa).
通过亲和色谱法从牛肝脏中获得了针对固定在琼脂糖凝胶柱上的酪氨酰 - tRNA合成酶（TyrRS）纯化的多克隆抗体。研究了这些抗体的免疫特异性及其对牛肝脏中TyrRS酶活性的影响。我们发现多克隆抗体在同源tRNA（Tyr）的氨酰化反应中抑制TyrRS酶活性达47%。免疫印迹显示，这些抗体既与天然酶（分子量2.59 kDa）反应，也与酶的蛋白水解裂解的功能活性形式（分子量2.39 kDa）反应。
Zotero 插件