Koya Y, Matsubara T, Ikeuchi T, Adachi S, Yamauchi K
Faculty of Education, Gifu University, Japan.
Comp Biochem Physiol A Physiol. 1997 Dec;118(4):1217-23. doi: 10.1016/s0300-9629(97)00042-x.
Vitellogenin of matrotrophic viviparous eelpout (Zoarces elongatus) was purified from estradiol-17 beta (E2) treated immature male sera by gel chromatography and anion exchange chromatography. Isolated vitellogenin has a molecular weight of 540 kDa estimated by gel chromatography. Serum levels of vitellogenin in females were measured during oocyte development and gestation by single radial immunodiffusion. Serum vitellogenin level was low (less than 0.2 mg/ml) during the early vitellogenic period, increased in the late vitellogenic period to a peak level (6.4 +/- 2.1 mg/ml) at the beginning of gestation. After that it rapidly decreased to a low level (0.1 +/- 0.1 mg/ml) during the early gestation period. Levels of vitellogenin remained low throughout the gestation period. Serum E2 levels in females showed increased from 1.3 to 3.0 ng/ml during the late vitellogenic period, and declined to 0.4 ng/ml during the early gestation period. Serum levels of E2 showed good correlation with serum vitellogenin levels, suggesting that the vitellogenin synthesis is controlled by E2 in this species. These results combined with the matrotrophic growth of embryo during gestation suggest that there is a shift in the synthesis of maternal nutritional products for embryos from the yolk to other nutrients.
通过凝胶色谱法和阴离子交换色谱法，从经17β-雌二醇（E2）处理的未成熟雄性血清中纯化出了胎生胎养型长蛇绵鳚（Zoarces elongatus）的卵黄蛋白原。通过凝胶色谱法估算，分离出的卵黄蛋白原分子量为540 kDa。采用单向免疫扩散法，在卵母细胞发育和妊娠期间测定了雌性血清中的卵黄蛋白原水平。在卵黄生成早期，血清卵黄蛋白原水平较低（低于0.2 mg/ml），在卵黄生成后期升高，在妊娠开始时达到峰值水平（6.4±2.1 mg/ml）。此后，在妊娠早期迅速降至低水平（0.1±0.1 mg/ml）。在整个妊娠期，卵黄蛋白原水平一直保持较低。雌性血清E2水平在卵黄生成后期从1.3 ng/ml升至3.0 ng/ml，在妊娠早期降至0.4 ng/ml。血清E2水平与血清卵黄蛋白原水平显示出良好的相关性，表明该物种中卵黄蛋白原的合成受E2控制。这些结果与妊娠期胚胎的胎生胎养生长相结合，表明母体为胚胎提供营养产物的合成从卵黄向其他营养物质发生了转变。
