血清素、P物质和甲硫氨酸脑啡肽-精氨酸6-甘氨酸7-亮氨酸8在反刍动物十二指肠内分泌细胞中的免疫组织化学共定位
Immunohistochemical colocalization of serotonin, substance P and Met-enkephalin-Arg6-Gly7-Leu8 in the endocrine cells of the ruminant duodenum.
作者信息
Mimoda T, Kitamura N, Hondo E, Yamada J
机构信息
Department of Veterinary Anatomy, Obihiro University of Agriculture and Veterinary Medicine, Japan.
出版信息
Anat Histol Embryol. 1998 Feb;27(1):65-9. doi: 10.1111/j.1439-0264.1998.tb00158.x.
The duodenum of various ruminants was examined by immunohistochemical staining for serotonin, substance P and Metenkephalin-Arg6-Gly7-Leu8 (MENK8). Serotonin- and substance P-immunoreactive endocrine cells were detected in samples from cow, calf, sheep, goat, and barbary sheep, MENK8-immunoreactive endocrine cells were detected exclusively in samples from cow and calf. Substance P and MENK8 immunoreactivity was found in serotonin-immunoreactive endocrine cells of cattle. Substance P- and MENK8-immunoreactive nervous elements were detected in all ruminants examined. The present results suggest that the expression and/or the mechanism that controls the expression of each peptide might differ between endocrine cells and nerve cells and might also depend on animal species.
通过免疫组织化学染色法，对各种反刍动物的十二指肠进行了5-羟色胺、P物质和甲硫氨酸脑啡肽-精氨酸6-甘氨酸7-亮氨酸8（MENK8）检测。在牛、犊牛、绵羊、山羊和蛮羊的样本中检测到了5-羟色胺和P物质免疫反应性内分泌细胞，仅在牛和犊牛的样本中检测到了MENK8免疫反应性内分泌细胞。在牛的5-羟色胺免疫反应性内分泌细胞中发现了P物质和MENK8免疫反应性。在所有检测的反刍动物中均检测到了P物质和MENK8免疫反应性神经成分。目前的结果表明，每种肽的表达和/或控制其表达的机制可能在内分泌细胞和神经细胞之间存在差异，并且可能还取决于动物种类。
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