细胞器膜融合：Syntaxin 同源物 Ufe1p 在 ER 膜融合中的新功能。
Organelle membrane fusion: a novel function for the syntaxin homolog Ufe1p in ER membrane fusion.
作者信息
Patel S K, Indig F E, Olivieri N, Levine N D, Latterich M
机构信息
The Salk Institute, La Jolla, California 92037, USA.
出版信息
Cell. 1998 Mar 6;92(5):611-20. doi: 10.1016/s0092-8674(00)81129-0.
The fusion of endoplasmic reticulum (ER) membranes in yeast does not require Sec18p/NSF and Sec17p, two proteins needed for docking of vesicles with their target membrane. Instead, ER membranes require a NSF-related ATPase, Cdc48p. Since both vesicular and organelle fusion events use related ATPases, we investigated whether both fusion events are also SNARE mediated. We present evidence that the fusion of ER membranes requires Ufe1p, a t-SNARE that localizes to the ER, but no known v-SNAREs. We propose that the Ufe1 protein acts in the dual capacity of an organelle membrane fusion-associated SNARE by undergoing direct t-t-SNARE and Cdc48p interactions during organelle membrane fusion as well as a t-SNARE for vesicular traffic.
酵母内质网（ER）膜的融合不需要Sec18p/NSF和Sec17p，这两种蛋白质是囊泡与靶膜对接所必需的。相反，内质网膜需要一种与NSF相关的ATP酶Cdc48p。由于囊泡和细胞器融合事件都使用相关的ATP酶，我们研究了这两种融合事件是否也由SNARE介导。我们提供的证据表明，内质网膜的融合需要Ufe1p，一种定位于内质网的t-SNARE，但不需要已知的v-SNARE。我们提出，Ufe1蛋白在细胞器膜融合相关SNARE中具有双重作用，即在细胞器膜融合过程中通过直接的t-t-SNARE和Cdc48p相互作用发挥作用，同时也是囊泡运输的t-SNARE。
Zotero 插件