Lin D C, Grossman A D
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Cell. 1998 Mar 6;92(5):675-85. doi: 10.1016/s0092-8674(00)81135-6.
We have identified a DNA site involved in chromosome partitioning in B. subtilis. This site was identified in vivo as the binding site for the chromosome partitioning protein Spo0J, a member of the ParB family of partitioning proteins. Spo0J is a site-specific DNA-binding protein that recognizes a 16 bp sequence found in spo0J. Allowing two mismatches, this sequence occurs ten times in the entire B. subtilis chromosome, all in the origin-proximal approximately 20%. Eight of the ten sequences are bound to Spo0J in vivo. The presence of a site on an otherwise unstable plasmid stabilized the plasmid in a Spo0J-dependent manner, demonstrating that this site, called parS, can function as a partitioning site. This site and Spo0J are conserved in a wide range of bacterial species.
我们已经鉴定出一个参与枯草芽孢杆菌染色体分配的DNA位点。该位点在体内被鉴定为染色体分配蛋白Spo0J的结合位点，Spo0J是ParB家族分配蛋白的成员之一。Spo0J是一种位点特异性DNA结合蛋白，可识别spo0J中发现的16bp序列。允许两个错配，该序列在整个枯草芽孢杆菌染色体中出现十次，全部位于近端约20%的区域。十个序列中的八个在体内与Spo0J结合。在其他不稳定质粒上存在该位点以Spo0J依赖的方式稳定了质粒，表明这个被称为parS的位点可以作为一个分配位点发挥作用。该位点和Spo0J在广泛的细菌物种中是保守的。
