腺病毒12型在KB细胞生产性感染期间的特异性RNA序列。
Adenovirus type 12-specific RNA sequences during productive infection of KB cells.
作者信息
Smiley J R, Mak S
出版信息
J Virol. 1976 Jul;19(1):36-42. doi: 10.1128/JVI.19.1.36-42.1976.
Abstract
The complementary strands of adenovirus type 12 DNA were separated, and virus-specific RNA was analyzed by saturation hybridization in solution. Late during infection whole cell RNA hybridized to 75% of the light (1) strand and 15% of the heavy (H) strand, whereas cytoplasmic RNA hybridized to 65% of the 1 strand and 15% of the h strand. Late nuclear RNA hybridized to about 90% of the 1 strand and at least 36% of the h strand. Double-stranded RNA was isolated from infected cells late after infection, which annealed to greater than 30% of each of the two complementary DNA strands. Early whole cell RNA hybridized to 45 to 50% of the 1 strand and 15% of the h strand, whereas early cytoplasmic RNA hybridized to about 15% of each of the complementary strands. All early cytoplasmic sequences were present in the cytoplasm at late times.
摘要
12型腺病毒DNA的互补链被分离出来，病毒特异性RNA通过溶液中的饱和杂交进行分析。在感染后期，全细胞RNA与轻链（1）的75%和重链（H）的15%杂交，而细胞质RNA与1链的65%和h链的15%杂交。后期核RNA与1链的约90%和h链的至少36%杂交。感染后期从感染细胞中分离出双链RNA，其与两条互补DNA链中每条链的30%以上退火。早期全细胞RNA与1链的45%至50%和h链的15%杂交，而早期细胞质RNA与每条互补链的约15%杂交。所有早期细胞质序列在后期都存在于细胞质中。
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