Jałoszyński P, Kujawski M, Czub-Swierczek M, Markowska J, Szyfter K
Institute of Human Genetics, Polish Academy of Sciences, Poznań.
Mutat Res. 1997 Dec;385(3):223-33. doi: 10.1016/s0921-8777(97)00046-3.
The studies concerned the response to bleomycin treatment in peripheral blood lymphocytes (PBL) of breast cancer (BC) subjects. The level of BLM-induced DNA strand breaks was evaluated using alkaline comet assay followed by visual scoring. The sensitivity to genotoxic exposure as well as the time-course of damage removal were estimated and analysed in comparison to control (healthy) subjects. Despite high inter-individual variability, the differences between the BC and non-cancer groups still proved to be statistically significant. Lymphocytes of the BC subjects appeared to be more sensitive to BLM exposure as shown by higher level of DNA damage. The DNA repair capacity was weaker in PBL obtained from BC patients than that in lymphocytes of controls.
这些研究关注了乳腺癌（BC）患者外周血淋巴细胞（PBL）对博来霉素治疗的反应。使用碱性彗星试验并通过视觉评分来评估博来霉素诱导的DNA链断裂水平。与对照（健康）受试者相比，估计并分析了对基因毒性暴露的敏感性以及损伤清除的时间进程。尽管个体间存在高度变异性，但BC组和非癌症组之间的差异仍被证明具有统计学意义。如DNA损伤水平较高所示，BC患者的淋巴细胞对博来霉素暴露似乎更敏感。从BC患者获得的PBL中的DNA修复能力比对照组淋巴细胞中的弱。
