Becker E L, Forouhar F A, Grunnet M L, Boulay F, Tardif M, Bormann B J, Sodja D, Ye R D, Woska J R, Murphy P M
Department of Pathology, University of Connecticut Health Center Farmington, 263 Farmington Avenue, Farmington, CT 06030-3105, USA.
Cell Tissue Res. 1998 Apr;292(1):129-35. doi: 10.1007/s004410051042.
The formylpeptide receptor (FPR), previously found only on polymorphonuclear leukocytes and monocytes/macrophages, responds to both synthetic N-formyl oligopeptides and those produced by bacteria. The cDNA for human FPR has been cloned and a rabbit polyclonal antiserum directed against a synthetic 11-amino-acid peptide corresponding to the deduced carboxy-terminus has been produced. We have now extensively characterized and used the antibody to detect FPR on normal human tissues and cell types. The receptor antigen is present on some epithelial cells, especially those with a secretory function, and on some endocrine cells, e.g., follicular cells of the thyroid and cortical cells of the adrenal. Liver hepatocytes and Kupffer cells are positive. Smooth muscle and endothelial cells are also generally positive. In the brain and spinal cord, the neurons of the motor, sensory, and cerebellar systems, and those of the parasympathetic and sympathetic systems stain positively. These data suggest that the putative endogenous agonist for FPR or an antigenically similar receptor reacts with cellular targets in the neuromuscular, vascular, endocrine, and immune systems.
甲酰肽受体（FPR）以前仅在多形核白细胞和单核细胞/巨噬细胞上发现，它对合成的N-甲酰寡肽和细菌产生的寡肽均有反应。人FPR的cDNA已被克隆，并制备了一种兔多克隆抗血清，该抗血清针对与推导的羧基末端相对应的合成11氨基酸肽。我们现在已经对该抗体进行了广泛的表征，并使用它来检测正常人组织和细胞类型上的FPR。受体抗原存在于一些上皮细胞上，特别是那些具有分泌功能的上皮细胞，以及一些内分泌细胞上，例如甲状腺的滤泡细胞和肾上腺的皮质细胞。肝肝细胞和枯否细胞呈阳性。平滑肌和内皮细胞通常也呈阳性。在脑和脊髓中，运动、感觉和小脑系统的神经元，以及副交感和交感系统的神经元染色呈阳性。这些数据表明，FPR或抗原相似受体的假定内源性激动剂与神经肌肉、血管、内分泌和免疫系统中的细胞靶点发生反应。
