Sonntag J, Brandenburg U, Polzehl D, Strauss E, Vogel M, Dudenhausen J W, Obladen M
Department of Neonatology, Charité-Virchow-Hospital, Humboldt University Berlin, Augustenburger Platz 1, D-13353 Berlin, Germany.
Pediatr Dev Pathol. 1998 Mar-Apr;1(2):131-5. doi: 10.1007/s100249900016.
Activation of the complement system occurs in several diseases. For reliable identification of complement activation in neonates, we establish reference ranges of several components in cord blood of healthy term newborns. For this study, cord blood samples were taken from 125 healthy term newborns. Concentrations of C1r, C2, C5, C7, Properdin, and factors D, H, and I were determined by single radial immunodiffusion. C3a and C5a were measured by specific EIA and complement function was measured by hemolytic assays. The results were expressed as 5th percentile, median, and 95th percentile. The following respective concentrations were found: C1r: 27, 47, 65 mg/l; C2: 12.0, 18.0, 24.0 mg/l; C5: 64, 92, 127 mg/l; C7: 32, 60, 89 mg/l; Properdin: 5.6, 9.7, 14.2 mg/l; factor D: 3.6, 5.2, 7.3 mg/l; factor H: 178, 234, 296 mg/l; and factor I: 15, 24, 32 mg/l. The functional activity of the whole complement system was 24%, 43%, 97% and for the alternative pathway 39%, 58%, 76%. The concentration of the activated split products C3a was 4, 65, 255 microg/l and of C5a, 0.11, 0.26, 1.19 microg/l. These reference values may be important for the detection of deficiencies of native complement proteins or perinatal processes leading to an activation of the complement system.
补体系统的激活在多种疾病中都会发生。为了可靠地识别新生儿中的补体激活情况，我们建立了健康足月儿脐带血中几种补体成分的参考范围。在本研究中，采集了125名健康足月儿的脐带血样本。通过单向放射免疫扩散法测定C1r、C2、C5、C7、备解素以及D因子、H因子和I因子的浓度。通过特异性酶免疫测定法检测C3a和C5a，并通过溶血试验检测补体功能。结果以第5百分位数、中位数和第95百分位数表示。得到了以下各自的浓度：C1r：27、47、65mg/l；C2：12.0、18.0、24.0mg/l；C5：64、92、127mg/l；C7：32、60、89mg/l；备解素：5.6、9.7、14.2mg/l；D因子：3.6、5.2、7.3mg/l；H因子：178、234、296mg/l；I因子：15、24、32mg/l。整个补体系统的功能活性分别为24%、43%、97%，替代途径的功能活性分别为39%、58%、76%。活化裂解产物C3a的浓度为4、65、255μg/l，C5a的浓度为0.11、0.26、1.19μg/l。这些参考值对于检测天然补体蛋白缺乏或导致补体系统激活的围产期过程可能具有重要意义。
