Takemura M, Tsujio A, Iwase K, Shimada T, Shigenaga Y
Department of Oral Anatomy, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565, Japan.
Brain Res. 1998 Jan 19;781(1-2):78-90. doi: 10.1016/s0006-8993(97)01210-9.
Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) activity and the central terminal fields of branches of the mandibular and chorda tympani nerves were visualized histochemically at the same time using transganglionic transport of wheat germ agglutinin conjugated with horseradish peroxidase. The blue NADPH-d-positive neurons comprised a sparse network in the dorsomedial spinal trigeminal subnucleus oralis and a dense one in the rostral lateral division of the nucleus of the solitary tract. In the subnucleus caudalis, most labeled neurons were in the superficial zone, and smaller numbers were in the magnocellular zone. The NADPH-d-positive neurons in the subnucleus oralis and the nucleus of the solitary tract overlapped mostly with the transganglionically labeled terminal field from the lingual nerve, partly with the terminal field from the inferior alveolar and chorda tympani nerves, and rarely with the terminal field from the mental nerve. The NADPH-d-positive neurons in the dorsomedial paratrigeminal nucleus and subnucleus caudalis overlapped mostly with the terminal field from the lingual nerve, partly with the terminal field from the inferior alveolar and mental nerves and never with the terminal field from the chorda tympani. A statistically significant reduction in the number of NADPH-d-positive neurons was seen bilaterally in subnucleus oralis and the nucleus of the solitary tract when the lingual nerve was transected. Inflammatory insults to the lingual nerve or tooth pulps significantly increased the number of NADPH-d-positive neurons in subnucleus oralis, the nucleus of the solitary tract, and subnucleus caudalis. These results show that the NO/cyclic GMP system in the trigeminal and solitary nuclei is differentially regulated trans-synaptically by trigeminal afferents depending on the nucleus and sensory modality.
利用与辣根过氧化物酶结合的小麦胚芽凝集素的跨神经节运输，同时通过组织化学方法观察了磷酸烟酰胺腺嘌呤二核苷酸黄递酶（NADPH-d）活性以及下颌神经和鼓索神经分支的中枢终末场。蓝色的NADPH-d阳性神经元在下颌神经节口侧背内侧亚核中构成稀疏网络，在孤束核嘴侧外侧部构成密集网络。在尾侧亚核中，大多数标记神经元位于浅区，少数位于大细胞区。口侧亚核和孤束核中的NADPH-d阳性神经元大多与来自舌神经的跨神经节标记终末场重叠，部分与来自下牙槽神经和鼓索神经的终末场重叠，很少与来自颏神经的终末场重叠。三叉旁背内侧核和尾侧亚核中的NADPH-d阳性神经元大多与来自舌神经的终末场重叠，部分与来自下牙槽神经和颏神经的终末场重叠，从不与来自鼓索神经的终末场重叠。切断舌神经后，双侧口侧亚核和孤束核中NADPH-d阳性神经元数量出现统计学显著减少。对舌神经或牙髓的炎性损伤显著增加了口侧亚核、孤束核和尾侧亚核中NADPH-d阳性神经元的数量。这些结果表明，三叉神经核和孤束核中的NO/环鸟苷酸系统通过三叉神经传入纤维跨突触地受到不同调节，这取决于核团和感觉模式。
