Granata A R, Ruggiero D A
Division of Neurobiology, Department of Neurology and Neuroscience, Cornell University Medical College, New York, NY 10021, USA.
Brain Res. 1998 Jan 19;781(1-2):329-34. doi: 10.1016/s0006-8993(97)01235-3.
Sympathetic outflow is regulated by a direct pathway of the rostral ventrolateral reticular formation (rvlm) to the thoracic spinal cord. For the first time, a dual retrograde/anterograde transport technique was used to demonstrate by light microscopy, potential disynaptic pathways from the rvlm to the thoracic spinal cord in the rat. An anterograde tracer, biotinylated dextran amine (BDA) was injected into the rvlm and a retrograde tracer, FluoroGold (FG) deposited into the upper thoracic spinal cord in the same animal. Rostral ventrolateral medullary efferents labeled with BDA were apposed to thoracic reticulospinal neurons labeled with FG in the ventrolateral tegmentum, ipsilateral and contralateral to the injection site in the rvlm. Suggestive evidence was obtained of synaptic interactions with neuronal somata and proximal dendrites. The results support the idea that the rvlm projects to the thoracic cord via disynaptic, intrareticular pathways paralleling the well established monosynaptic projection.
交感神经传出由延髓头端腹外侧网状结构（rvlm）至胸段脊髓的直接通路调节。首次采用双逆行/顺行转运技术，通过光学显微镜证实大鼠中从rvlm至胸段脊髓的潜在双突触通路。将顺行示踪剂生物素化葡聚糖胺（BDA）注入rvlm，并将逆行示踪剂荧光金（FG）注入同一只动物的上胸段脊髓。用BDA标记的延髓头端腹外侧传出纤维与在rvlm注射部位同侧和对侧的腹外侧被盖区中用FG标记的胸段网状脊髓神经元相邻。获得了与神经元胞体和近端树突突触相互作用的提示性证据。结果支持这样的观点，即rvlm通过与已确立的单突触投射平行的双突触、网状内通路投射至胸段脊髓。
