Harp T K, Hammond E G
Department of Food Science and Human Nutrition, Iowa State University, Ames 50011, USA.
Lipids. 1998 Feb;33(2):209-16. doi: 10.1007/s11745-998-0197-7.
Thirty soybean germplasm lines representing a wide distribution of fatty acid compositions were analyzed stereospecifically by using a chiral column to resolve the sn-1 and sn-3 positions of glycerol. The amounts of each acyl group on each of the sn positions were plotted vs. the amount of that acyl group in the triacylglycerols (TAG), and the plots were fitted by linear regression. The deviation of individual data points from the linear regressions was much greater than observed in previous studies. This could be attributed to the inclusion of a number of germplasm lines with elevated or reduced percentages of saturates. The stereospecific distributions could not be fit with previously suggested mathematical models because the plots had intercepts that were not allowed by the models. Statistical tests of the analytical procedure indicated that slight oxidation of or bias against the polyunsaturates had occurred and that the Grignard deacylation method gave slightly less representative analyses of the sn-2 position than pancreatic lipase deacylation on these TAG.
利用手性柱解析甘油的sn-1和sn-3位，对30个代表脂肪酸组成广泛分布的大豆种质系进行了立体专一性分析。将每个sn位上各酰基的含量与三酰甘油（TAG）中该酰基的含量作图，并通过线性回归对这些图进行拟合。与之前的研究相比，单个数据点与线性回归的偏差要大得多。这可能归因于纳入了一些饱和脂肪酸百分比升高或降低的种质系。立体专一性分布无法用先前提出的数学模型拟合，因为这些图的截距不符合模型要求。对分析程序的统计检验表明，多不饱和脂肪酸发生了轻微氧化或存在偏差，并且在这些TAG上，格氏脱酰法对sn-2位的分析代表性略低于胰脂肪酶脱酰法。
