用于测定日内瓦分枝杆菌克拉霉素敏感性的标准化BACTEC方法。
Standardized BACTEC method to measure clarithromycin susceptibility of Mycobacterium genavense.
作者信息
Carlson L D, Wallis C K, Coyle M B
机构信息
Department of Laboratory Medicine, Harborview Medical Center, University of Washington, Seattle 98104, USA.
出版信息
J Clin Microbiol. 1998 Mar;36(3):748-51. doi: 10.1128/JCM.36.3.748-751.1998.
Abstract
A standardized clarithromycin susceptibility test for Mycobacterium genavense is reported. The BACTEC radiometric broth dilution test method recommended for Mycobacterium avium complex was modified to develop a reliable and reproducible procedure. Test development involved optimization of medium pH and inoculum densities for antibiotic vials as well as growth control vials. MIC control organisms included Mycobacterium simiae, Mycobacterium avium, and Mycobacterium xenopi. Growth control vials required two to three inoculum dilutions, which varied for each species. Clarithromycin MICs and MBCs for 12 isolates and 1 colonial variant of M. genavense ranged from < or = 0.06 to 0.25 microg/ml.
摘要
报道了一种针对日内瓦分枝杆菌的标准化克拉霉素药敏试验。对推荐用于鸟分枝杆菌复合群的BACTEC放射测量肉汤稀释试验方法进行了改进，以建立一种可靠且可重复的程序。试验开发涉及优化抗生素瓶以及生长对照瓶的培养基pH值和接种密度。MIC对照菌株包括猿猴分枝杆菌、鸟分枝杆菌和偶发分枝杆菌。生长对照瓶需要两到三次接种物稀释，每种菌株各不相同。12株日内瓦分枝杆菌分离株和1株菌落变异株的克拉霉素MIC和MBC范围为≤0.06至0.25微克/毫升。
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